Restriction map for
pFN21AE5539
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 706 GGCGTC G[GA]^CG[CT]C
ApaI 422 GGGCCC GGGCC^C
AvaI 1114 CTCGAG C^[CT]CG[GA]G
BalI 872 TGGCCA TGG^CCA
BamHI 726 GGATCC G^GATCC
BbsI 709 GTCTTC GAAGAC(2/6)
BcnI 288 CCCGG CC^[GC]GG
BglII 155 AGATCT A^GATCT
BpuAI 709 GTCTTC GAAGAC(2/6)
BsaHI 706 GGCGTC G[GA]^CG[CT]C
BsaI 733 GAGACC GGTCTC(1/5)
BsmFI 128 GTCCC GGGAC(10/14)
BsmI 532 GCATTC GAATGC(1/-1)
BsoBI 1114 CTCGAG C^[CT]CG[GA]G
BsrDI 580 GCAATG GCAATG(2/0)
BsrI 374 ACTGG ACTGG(1/-1)
BstEII 810 GGTCACC G^GT[ACGT]ACC
BstF5I 75 CATCC GGATG(2/0)
BstPI 810 GGTCACC G^GT[ACGT]ACC
CfoI 397 GCGC GCG^C
EarI 699 GAAGAG CTCTTC(1/4)
EcoO65I 810 GGTCACC G^GT[ACGT]ACC
EcoRI 313 GAATTC G^AATTC
EcoT22I 530 ATGCAT ATGCA^T
FokI 75 CATCC GGATG(9/13)
HaeII 396 GGCGCT [GA]GCGC^[CT]
HapII 289 CCGG C^CGG
HhaI 397 GCGC GCG^C
Hin1I 706 GGCGTC G[GA]^CG[CT]C
HinP1I 397 GCGC G^CGC
HpaII 289 CCGG C^CGG
Ksp632I 699 GAAGAG CTCTTC(1/4)
MaeII 887 ACGT A^CGT
MluNI 872 TGGCCA TGG^CCA
MscI 872 TGGCCA TGG^CCA
MspA1I 232 CAGCTG C[AC]G^C[GT]G
MspI 289 CCGG C^CGG
NciI 288 CCCGG CC^[GC]GG
NcoI 662 CCATGG C^CATGG
NsiI 530 ATGCAT ATGCA^T
PaeR7I 1114 CTCGAG C^TCGAG
PpuMI 126 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 232 CAGCTG CAG^CTG
SspI 1010 AATATT AAT^ATT
XhoI 1114 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
781 GACATACTGTC
AlwI 727 GATCC GGATC(4/5)
726 GGATC
AspEI 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
781 GACATACTGTC
AspHI 187 GAGCAC G[AT]GC[AT]^C
325 GAGCAC
AvaII 127 GGTCC G^G[AT]CC
278 GGACC
BsgI 538 CTGCAC GTGCAG(16/14)
848 GTGCAG
BsiHKAI 187 GAGCAC G[AT]GC[AT]^C
325 GAGCAC
Bsp1407I 479 TGTACA T^GTACA
1031 TGTACA
BsrGI 479 TGTACA T^GTACA
1031 TGTACA
BstYI 155 AGATCT [GA]^GATC[CT]
726 GGATCC
DraI 610 TTTAAA TTT^AAA
1006 TTTAAA
DsaI 662 CCATGG C^C[GA][CT]GG
980 CCGTGG
Eam1105I 735 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
781 GACATACTGTC
FauI 359 CCCGC CCCGC(4/6)
911 CCCGC
HgaI 148 GACGC GACGC(5/10)
707 GCGTC
MflI 155 AGATCT [GA]^GATC[CT]
726 GGATCC
MseI 611 TTAA T^TAA
1007 TTAA
MslI 328 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
739 CATTGTCATG
PleI 411 GAGTC GAGTC(4/5)
963 GAGTC
PstI 402 CTGCAG CTGCA^G
1102 CTGCAG
SfcI 402 CTGCAG C^T[GA][CT]AG
1102 CTGCAG
SmlI 132 CTCAAG C^T[CT][GA]AG
1114 CTCGAG
SspBI 479 TGTACA T^GTACA
1031 TGTACA
Tru9I 611 TTAA T^TAA
1007 TTAA
TspRI 23 CAGTG CA[GC]TG[ACGT][ACGT]^
483 CAGTG
XcmI 496 CCATTCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1048 CCATTCAAGCCTTGG
XhoII 155 AGATCT [GA]^GATC[CT]
726 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbvI BciVI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SexAI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TthHB8I