Restriction map for
pFN21AE5350
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AcsI AfaI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstYI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 709 GACGTC GACGT^C
AcyI 709 GACGTC G[GA]^CG[CT]C
AflIII 544 ACATGT A^C[GA][CT]GT
AhdI 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 742 GGATC GGATC(4/5)
AspEI 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 242 TGATCA T^GATCA
BfaI 914 CTAG C^TAG
BlpI 64 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 64 GCTGAGC GC^T[ACGT]AGC
BsaHI 709 GACGTC G[GA]^CG[CT]C
BsmAI 539 GAGAC GTCTC(1/5)
BsmI 562 GCATTC GAATGC(1/-1)
BspLU11I 544 ACATGT A^CATGT
BsrFI 763 ACCGGC [GA]^CCGG[CT]
BsrI 16 CCAGT ACTGG(1/-1)
BstAPI 780 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 816 GGTGACC G^GT[ACGT]ACC
BstPI 816 GGTGACC G^GT[ACGT]ACC
CelII 64 GCTGAGC GC^T[ACGT]AGC
Cfr10I 763 ACCGGC [GA]^CCGG[CT]
DsaI 518 CCATGG C^C[GA][CT]GG
Eam1105I 716 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 816 GGTGACC G^GT[ACGT]ACC
FbaI 242 TGATCA T^GATCA
Hin1I 709 GACGTC G[GA]^CG[CT]C
MaeI 914 CTAG C^TAG
MboII 323 TCTTC GAAGA(8/7)
MseI 115 TTAA T^TAA
NcoI 518 CCATGG C^CATGG
PflMI 513 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 79 GATGC GCATC(5/9)
SmaI 173 CCCGGG CCC^GGG
SphI 216 GCATGC GCATG^C
Tru9I 115 TTAA T^TAA
Van91I 513 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 518 CCATGGCTAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 173 CCCGGG C^CCGGG
XmaI 173 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 694 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
790 CAGGAGCTG
AvaI 173 CCCGGG C^[CT]CG[GA]G
619 CCCGAG
BalI 103 TGGCCA TGG^CCA
638 TGGCCA
BanII 67 GAGCCC G[GA]GC[CT]^C
684 GGGCTC
BsoBI 173 CCCGGG C^[CT]CG[GA]G
619 CCCGAG
BspMI 408 ACCTGC ACCTGC(4/8)
820 ACCTGC
BstXI 338 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
761 CCACCGGCTTGG
DpnII 243 GATC ^GATC
743 GATC
HphI 816 GGTGA GGTGA(8/7)
758 TCACC
MboI 243 GATC ^GATC
743 GATC
MluNI 103 TGGCCA TGG^CCA
638 TGGCCA
MscI 103 TGGCCA TGG^CCA
638 TGGCCA
MslI 217 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
234 CACCTATGTG
MspA1I 30 CAGCTG C[AC]G^C[GT]G
634 CAGCTG
NdeII 243 GATC ^GATC
743 GATC
PleI 10 GACTC GAGTC(4/5)
89 GACTC
PvuII 30 CAGCTG CAG^CTG
634 CAGCTG
Sau3AI 243 GATC ^GATC
743 GATC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeII MaeIII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV NspI PpuMI PstI Sau96I
ScrFI SfcI StyI TfiI TseI
Tsp45I TspRI