Restriction map for
pFN21AE2675
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRII EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvaI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TspRI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AocI 184 CCTAAGG CC^T[ACGT]AGG
AspI 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 6 GGCGCC G^G[CT][GA]CC
BanII 43 GGGCTC G[GA]GC[CT]^C
BbeI 6 GGCGCC GGCGC^C
BcgI 109 GCATTTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 147 CCGGG CC^[GC]GG
BmyI 43 GGGCTC G[AGT]GC[ACT]^C
BpmI 450 CTGGAG CTGGAG(16/14)
BseRI 193 GAGGAG GAGGAG(10/8)
BsmAI 378 GAGAC GTCTC(1/5)
Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 298 TGTACA T^GTACA
BspHI 918 TCATGA T^CATGA
BsrGI 298 TGTACA T^GTACA
BssKI 147 CCGGG ^CC[ACGT]GG
BstAPI 778 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 249 GGATG GGATG(2/0)
BstXI 68 CCATGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 184 CCTAAGG CC^T[ACGT]AGG
DsaI 68 CCATGG C^C[GA][CT]GG
EaeI 77 TGGCCG [CT]^GGCC[GA]
Eco81I 184 CCTAAGG CC^T[ACGT]AGG
EcoRV 872 GATATC GAT^ATC
FauI 48 CCCGC CCCGC(4/6)
FokI 249 GGATG GGATG(9/13)
HaeII 6 GGCGCC [GA]GCGC^[CT]
HgaI 136 GCGTC GACGC(5/10)
HincII 159 GTTGAC GT[CT]^[GA]AC
HindII 159 GTTGAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
NarI 6 GGCGCC GG^CGCC
NciI 147 CCGGG CC^[GC]GG
NcoI 68 CCATGG C^CATGG
PflFI 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 31 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 918 TCATGA T^CATGA
ScrFI 147 CCGGG CC^[ACGT]GG
SfcI 788 CTACAG C^T[GA][CT]AG
SfoI 6 GGCGCC GGC^GCC
SspBI 298 TGTACA T^GTACA
TaqI 270 TCGA T^CGA
Tth111I 115 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 270 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 6 GGCGCC G[GA]^CG[CT]C
135 GGCGTC
AvaII 32 GGACC G^G[AT]CC
92 GGACC
BglII 627 AGATCT A^GATCT
901 AGATCT
BsaHI 6 GGCGCC G[GA]^CG[CT]C
135 GGCGTC
BsaJI 68 CCATGG C^C[ACGT][ACGT]GG
74 CCTTGG
BsiYI 35 CCCAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
488 CCTCTTCATGG
BslI 35 CCCAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
488 CCTCTTCATGG
BsmFI 91 GGGAC GGGAC(10/14)
396 GTCCC
BstYI 627 AGATCT [GA]^GATC[CT]
901 AGATCT
Cac8I 221 GCAGGC GC[ACGT]^[ACGT]GC
334 GCTTGC
CfoI 7 GCGC GCG^C
652 GCGC
DraII 31 AGGACCC [GA]G^G[ACGT]CC[CT]
930 AGGGCCT
EarI 489 CTCTTC CTCTTC(1/4)
190 GAAGAG
EcoO109I 31 AGGACCC [GA]G^G[ACGT]CC[CT]
930 AGGGCCT
EcoT14I 68 CCATGG C^C[AT][AT]GG
74 CCTTGG
HapII 80 CCGG C^CGG
147 CCGG
HhaI 7 GCGC GCG^C
652 GCGC
Hin1I 6 GGCGCC G[GA]^CG[CT]C
135 GGCGTC
HinP1I 7 GCGC G^CGC
652 GCGC
HpaII 80 CCGG C^CGG
147 CCGG
Ksp632I 489 CTCTTC CTCTTC(1/4)
190 GAAGAG
MaeIII 455 GTGAC ^GT[ACGT]AC
836 GTGAC
MflI 627 AGATCT [GA]^GATC[CT]
901 AGATCT
MspI 80 CCGG C^CGG
147 CCGG
NspI 205 GCATGT [GA]CATG^[CT]
295 GCATGT
PleI 115 GACTC GAGTC(4/5)
466 GACTC
PvuII 358 CAGCTG CAG^CTG
791 CAGCTG
SfaNI 402 GATGC GCATC(5/9)
698 GATGC
StyI 68 CCATGG C^C[AT][AT]GG
74 CCTTGG
Tsp45I 455 GTGAC ^GT[GC]AC
836 GTGAC
XhoII 627 AGATCT [GA]^GATC[CT]
901 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsrI Cfr13I DdeI DpnII
Fnu4HI HaeIII HinfI HphI ItaI
MboI MboII MnlI MseI MspA1I
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I TfiI Tru9I TseI
Tsp509I