Restriction map for
pFN21AE5657
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BfrI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AclI 962 AACGTT AA^CGTT
Aor51HI 127 AGCGCT AGC^GCT
Asp700I 737 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 915 GAGCAC G[AT]GC[AT]^C
AvaI 123 CCCGAG C^[CT]CG[GA]G
BciVI 636 GGATAC GTATCC(6/5)
BclI 1416 TGATCA T^GATCA
BglI 41 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 518 CTGGAG CTGGAG(16/14)
BsgI 503 CTGCAC GTGCAG(16/14)
BsiHKAI 915 GAGCAC G[AT]GC[AT]^C
BsmI 741 GCATTC GAATGC(1/-1)
BsoBI 123 CCCGAG C^[CT]CG[GA]G
BspHI 1422 TCATGA T^CATGA
BsrBI 1098 CCGCTC CCGCTC(-3/-3)
BssSI 227 CTCGTG CACGAG(-5/-1)
DraII 39 GGGCCCC [GA]G^G[ACGT]CC[CT]
Eco47III 127 AGCGCT AGC^GCT
EcoO109I 39 GGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 1416 TGATCA T^GATCA
HincII 301 GTTGAC GT[CT]^[GA]AC
HindII 301 GTTGAC GT[CT]^[GA]AC
MseI 242 TTAA T^TAA
MslI 1434 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 45 CAGCGG C[AC]G^C[GT]G
NdeI 326 CATATG CA^TATG
NheI 130 GCTAGC G^CTAGC
Psp1406I 962 AACGTT AA^CGTT
RcaI 1422 TCATGA T^CATGA
ScaI 574 AGTACT AGT^ACT
Tru9I 242 TTAA T^TAA
XmnI 737 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 277 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
556 CAGTTGCTG
ApaI 39 GGGCCC GGGCC^C
52 GGGCCC
AvaII 34 GGTCC G^G[AT]CC
1218 GGACC
AvrII 165 CCTAGG C^CTAGG
1111 CCTAGG
BanI 91 GGCACC G^G[CT][GA]CC
375 GGCACC
BglII 880 AGATCT A^GATCT
1057 AGATCT
BlnI 165 CCTAGG C^CTAGG
1111 CCTAGG
BsaBI 749 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGATCATC
BsaI 610 GGTCTC GGTCTC(1/5)
1076 GGTCTC
BseRI 768 GAGGAG GAGGAG(10/8)
771 GAGGAG
BsmBI 819 GAGACG CGTCTC(1/5)
1026 GAGACG
BspMI 31 GCAGGT ACCTGC(4/8)
394 GCAGGT
BstYI 880 AGATCT [GA]^GATC[CT]
1057 AGATCT
HaeII 127 AGCGCT [GA]GCGC^[CT]
451 GGCGCT
HaeIII 40 GGCC GG^CC
53 GGCC
HindIII 359 AAGCTT A^AGCTT
565 AAGCTT
MamI 749 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGATCATC
MflI 880 AGATCT [GA]^GATC[CT]
1057 AGATCT
PshAI 111 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
821 GACGGCGGTC
PstI 29 CTGCAG CTGCA^G
548 CTGCAG
SfaNI 144 GATGC GCATC(5/9)
279 GCATC
TaqI 176 TCGA T^CGA
817 TCGA
Tsp509I 696 AATT ^AATT
1091 AATT
TthHB8I 176 TCGA T^CGA
817 TCGA
XbaI 790 TCTAGA T^CTAGA
1006 TCTAGA
XhoII 880 AGATCT [GA]^GATC[CT]
1057 AGATCT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FauI
Fnu4HI FokI HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI TseI Tsp45I TspRI