Restriction map for
pFN21AE6484
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BpmI BseAI BseRI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I Cfr10I CpoI DraII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoT22I FauI
FbaI FseI FspI HgaI HindIII
HpaI KspI MluI MroI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1197 GGTACC G^GTACC
AciI 1059 GCGG CCGC(-3/-1)
AcyI 821 GGCGCC G[GA]^CG[CT]C
AflIII 975 ACGTGT A^C[GA][CT]GT
Alw44I 110 GTGCAC G^TGCAC
ApaLI 110 GTGCAC G^TGCAC
Asp718I 1197 GGTACC G^GTACC
AspHI 110 GTGCAC G[AT]GC[AT]^C
AvaI 446 CCCGAG C^[CT]CG[GA]G
BbeI 821 GGCGCC GGCGC^C
BbvI 477 GCAGC GCAGC(8/12)
BlpI 1476 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1476 GCTAAGC GC^T[ACGT]AGC
BsaAI 974 TACGTG [CT]AC^GT[GA]
BsaBI 97 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 821 GGCGCC G[GA]^CG[CT]C
BsaI 779 GAGACC GGTCTC(1/5)
BsaWI 1718 ACCGGA [AT]^CCGG[AT]
BsiEI 1716 CGACCG CG[GA][CT]^CG
BsiHKAI 110 GTGCAC G[AT]GC[AT]^C
BsmFI 1175 GGGAC GGGAC(10/14)
BsoBI 446 CCCGAG C^[CT]CG[GA]G
BspDI 286 ATCGAT AT^CGAT
BsrDI 889 CATTGC GCAATG(2/0)
Bst1107I 1314 GTATAC GTA^TAC
BstEII 457 GGTAACC G^GT[ACGT]ACC
BstPI 457 GGTAACC G^GT[ACGT]ACC
BstXI 256 CCAACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1314 GTATAC GTA^TAC
CelII 1476 GCTAAGC GC^T[ACGT]AGC
ClaI 286 ATCGAT AT^CGAT
DraI 1263 TTTAAA TTT^AAA
DraIII 612 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1177 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1595 CCATGG C^C[GA][CT]GG
EcoO65I 457 GGTAACC G^GT[ACGT]ACC
EcoRV 1676 GATATC GAT^ATC
Fnu4HI 477 GCAGC GC^[ACGT]GC
HaeII 821 GGCGCC [GA]GCGC^[CT]
Hin1I 821 GGCGCC G[GA]^CG[CT]C
HincII 676 GTCAAC GT[CT]^[GA]AC
HindII 676 GTCAAC GT[CT]^[GA]AC
ItaI 477 GCAGC GC^[ACGT]GC
KasI 821 GGCGCC G^GCGCC
KpnI 1197 GGTACC GGTAC^C
MamI 97 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 574 CAATTG C^AATTG
MspA1I 122 CAGCTG C[AC]G^C[GT]G
MunI 574 CAATTG C^AATTG
MwoI 409 GCTGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 821 GGCGCC GG^CGCC
NcoI 1595 CCATGG C^CATGG
PvuII 122 CAGCTG CAG^CTG
SapI 1533 GAAGAGC GCTCTTC(1/4)
SfoI 821 GGCGCC GGC^GCC
TaqI 287 TCGA T^CGA
TseI 477 GCAGC G^C[AT]GC
TthHB8I 287 TCGA T^CGA
XbaI 1837 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 658 GTCTAC GT^[AC][GT]AC
1314 GTATAC
AcsI 82 AAATTT [GA]^AATT[CT]
1498 AAATTT
ApoI 82 AAATTT [GA]^AATT[CT]
1498 AAATTT
BalI 265 TGGCCA TGG^CCA
1380 TGGCCA
BanII 252 GAGCCC G[GA]GC[CT]^C
427 GAGCCC
BcnI 152 CCCGG CC^[GC]GG
1319 CCCGG
BglII 96 AGATCT A^GATCT
1432 AGATCT
BsmAI 779 GAGAC GTCTC(1/5)
232 GTCTC
BspHI 77 TCATGA T^CATGA
1118 TCATGA
CfoI 717 GCGC GCG^C
822 GCGC
EaeI 265 TGGCCA [CT]^GGCC[GA]
1380 TGGCCA
HhaI 717 GCGC GCG^C
822 GCGC
HinP1I 717 GCGC G^CGC
822 GCGC
HphI 136 GGTGA GGTGA(8/7)
683 TCACC
MaeII 201 ACGT A^CGT
975 ACGT
MluNI 265 TGGCCA TGG^CCA
1380 TGGCCA
MscI 265 TGGCCA TGG^CCA
1380 TGGCCA
NciI 152 CCCGG CC^[GC]GG
1319 CCCGG
RcaI 77 TCATGA T^CATGA
1118 TCATGA
SfcI 538 CTACAG C^T[GA][CT]AG
660 CTACAG
SmlI 1050 CTCAAG C^T[CT][GA]AG
1152 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AvaII
BanI BbsI BfaI BmyI BpuAI
BsaJI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FokI HaeIII HapII
HinfI HpaII Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
Tsp45I Tsp509I TspRI XhoII XmnI