Restriction map for
pFN21AE5339
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI NciI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 709 GACGTC GACGT^C
AluI 83 AGCT AG^CT
Alw44I 109 GTGCAC G^TGCAC
AlwI 210 GATCC GGATC(4/5)
AlwNI 162 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 109 GTGCAC G^TGCAC
AspHI 109 GTGCAC G[AT]GC[AT]^C
AvaI 8 CCCGAG C^[CT]CG[GA]G
BciVI 523 GGATAC GTATCC(6/5)
BfaI 448 CTAG C^TAG
BsiHKAI 109 GTGCAC G[AT]GC[AT]^C
BsoBI 8 CCCGAG C^[CT]CG[GA]G
Bsp1407I 284 TGTACA T^GTACA
BsrGI 284 TGTACA T^GTACA
BstF5I 782 GGATG GGATG(2/0)
BstXI 335 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 209 AGATCC [GA]^GATC[CT]
DsaI 335 CCATGG C^C[GA][CT]GG
EarI 190 CTCTTC CTCTTC(1/4)
EcoNI 393 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 782 GGATG GGATG(9/13)
HincII 154 GTCAAC GT[CT]^[GA]AC
HindII 154 GTCAAC GT[CT]^[GA]AC
Ksp632I 190 CTCTTC CTCTTC(1/4)
MaeI 448 CTAG C^TAG
MflI 209 AGATCC [GA]^GATC[CT]
MspA1I 165 CCGCTG C[AC]G^C[GT]G
NcoI 335 CCATGG C^CATGG
PleI 139 GACTC GAGTC(4/5)
SapI 189 GCTCTTC GCTCTTC(1/4)
SgrAI 246 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 284 TGTACA T^GTACA
TaqI 383 TCGA T^CGA
TfiI 515 GAATC G^A[AT]TC
TthHB8I 383 TCGA T^CGA
XcmI 161 CCAGCCGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 209 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AfaI 258 GTAC GT^AC
285 GTAC
BglI 13 GCCGCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
295 GCCGAGGTGGC
BmyI 109 GTGCAC G[AGT]GC[ACT]^C
264 GGGCAC
BpmI 141 CTCCAG CTGGAG(16/14)
121 CTGGAG
BseRI 37 CTCCTC GAGGAG(10/8)
391 CTCCTC
Bsp1286I 109 GTGCAC G[AGT]GC[ACT]^C
264 GGGCAC
BspHI 311 TCATGA T^CATGA
351 TCATGA
BsrFI 116 GCCGGC [GA]^CCGG[CT]
247 GCCGGC
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
247 GCCGGC
Cfr13I 442 GGGCC G^G[ACGT]CC
776 GGGCC
DdeI 418 CTGAG C^T[ACGT]AG
689 CTCAG
DpnII 210 GATC ^GATC
381 GATC
EcoT14I 197 CCAAGG C^C[AT][AT]GG
335 CCATGG
FauI 25 CCCGC CCCGC(4/6)
18 GCGGG
HapII 117 CCGG C^CGG
248 CCGG
HinfI 139 GACTC G^A[ACGT]TC
515 GAATC
HpaII 117 CCGG C^CGG
248 CCGG
MboI 210 GATC ^GATC
381 GATC
MseI 431 TTAA T^TAA
619 TTAA
MspI 117 CCGG C^CGG
248 CCGG
NaeI 116 GCCGGC GCC^GGC
247 GCCGGC
NdeII 210 GATC ^GATC
381 GATC
NgoMIV 116 GCCGGC G^CCGGC
247 GCCGGC
RcaI 311 TCATGA T^CATGA
351 TCATGA
RsaI 258 GTAC GT^AC
285 GTAC
Sau3AI 210 GATC ^GATC
381 GATC
Sau96I 442 GGGCC G^G[ACGT]CC
776 GGGCC
SfaNI 331 GATGC GCATC(5/9)
451 GCATC
StyI 197 CCAAGG C^C[AT][AT]GG
335 CCATGG
Tru9I 431 TTAA T^TAA
619 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AcsI AcyI ApoI
BanI BbeI BbvI BsaHI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstNI BstUI Cac8I CfoI EcoRII
Fnu4HI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HphI ItaI KasI
MaeII MboII MnlI MvaI MvnI
MwoI NarI NlaIII NlaIV ScrFI
SfoI TseI Tsp509I TspRI