Restriction map for
pFN21AE2568
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FbaI FokI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 331 CGCG CG^CG
AcsI 729 GAATTC [GA]^AATT[CT]
AlwI 234 GGATC GGATC(4/5)
AlwNI 444 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 591 GGGCCC GGGCC^C
ApoI 729 GAATTC [GA]^AATT[CT]
AvaI 595 CCCGGG C^[CT]CG[GA]G
AvaII 174 GGACC G^G[AT]CC
BanII 591 GGGCCC G[GA]GC[CT]^C
BbsI 382 GTCTTC GAAGAC(2/6)
BbvCI 427 GCTGAGG CCTCAGC(-5/-2)
BciVI 256 GGATAC GTATCC(6/5)
BfaI 148 CTAG C^TAG
BglII 287 AGATCT A^GATCT
BlpI 183 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 183 GCTCAGC GC^T[ACGT]AGC
BpuAI 382 GTCTTC GAAGAC(2/6)
BsmFI 539 GGGAC GGGAC(10/14)
BsoBI 595 CCCGGG C^[CT]CG[GA]G
BsrBI 181 CCGCTC CCGCTC(-3/-3)
BssSI 205 CACGAG CACGAG(-5/-1)
BstUI 331 CGCG CG^CG
BstYI 287 AGATCT [GA]^GATC[CT]
CelII 183 GCTCAGC GC^T[ACGT]AGC
DraII 591 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 75 CCACGG C^C[GA][CT]GG
EcoNI 759 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 591 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 729 GAATTC G^AATTC
EcoRV 577 GATATC GAT^ATC
EcoT14I 419 CCAAGG C^C[AT][AT]GG
MaeI 148 CTAG C^TAG
MaeIII 523 GTGAC ^GT[ACGT]AC
MflI 287 AGATCT [GA]^GATC[CT]
MslI 582 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 331 CGCG CG^CG
NspI 496 GCATGT [GA]CATG^[CT]
PshAI 310 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 190 CAGCTG CAG^CTG
SexAI 128 ACCTGGT A^CC[AT]GGT
SmaI 595 CCCGGG CCC^GGG
StyI 419 CCAAGG C^C[AT][AT]GG
Tsp45I 523 GTGAC ^GT[GC]AC
XcmI 443 CCAGCTTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 287 AGATCT [GA]^GATC[CT]
XmaCI 595 CCCGGG C^CCGGG
XmaI 595 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 64 GTGCAC G^TGCAC
119 GTGCAC
ApaLI 64 GTGCAC G^TGCAC
119 GTGCAC
AspHI 64 GTGCAC G[AT]GC[AT]^C
119 GTGCAC
BanI 347 GGCACC G^G[CT][GA]CC
695 GGCACC
BseRI 400 CTCCTC GAGGAG(10/8)
208 GAGGAG
BsgI 709 CTGCAC GTGCAG(16/14)
151 GTGCAG
BsiHKAI 64 GTGCAC G[AT]GC[AT]^C
119 GTGCAC
BsmBI 775 CGTCTC CGTCTC(1/5)
157 GAGACG
BsrFI 60 GCCGGT [GA]^CCGG[CT]
548 ACCGGC
Cfr10I 60 GCCGGT [GA]^CCGG[CT]
548 ACCGGC
DpnII 235 GATC ^GATC
288 GATC
FauI 180 CCCGC CCCGC(4/6)
83 GCGGG
HaeII 86 GGCGCT [GA]GCGC^[CT]
488 GGCGCT
HgaI 329 GACGC GACGC(5/10)
774 GCGTC
HinfI 101 GAGTC G^A[ACGT]TC
646 GAGTC
MboI 235 GATC ^GATC
288 GATC
MboII 383 TCTTC GAAGA(8/7)
608 TCTTC
NdeII 235 GATC ^GATC
288 GATC
PflMI 319 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
567 CCAGTGGGTGG
PleI 101 GAGTC GAGTC(4/5)
646 GAGTC
Sau3AI 235 GATC ^GATC
288 GATC
Tsp509I 601 AATT ^AATT
730 AATT
Van91I 319 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
567 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI
TspRI