##################################
# NO CUT ENZYMES (  224 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TaqI        
Tru9I       TseI        Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI           98  CCGC                 CCGC(-3/-1)
AcyI           76  GGCGTC               G[GA]^CG[CT]C
AflIII         62  ACGTGT               A^C[GA][CT]GT
AluI           11  AGCT                 AG^CT
BanII          55  GGGCTC               G[GA]GC[CT]^C
BmyI           55  GGGCTC               G[AGT]GC[ACT]^C
BsaAI          61  TACGTG               [CT]AC^GT[GA]
BsaHI          76  GGCGTC               G[GA]^CG[CT]C
BsmBI          93  CGTCTC               CGTCTC(1/5)
BsmFI          42  GTCCC                GGGAC(10/14)
Bsp1286I       55  GGGCTC               G[AGT]GC[ACT]^C
BspMI          34  ACCTGC               ACCTGC(4/8)
BsrI          105  CCAGT                ACTGG(1/-1)
BstXI          45  CCACAGGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         139  GATC                 ^GATC
HgaI           77  GCGTC                GACGC(5/10)
Hin1I          76  GGCGTC               G[GA]^CG[CT]C
HphI           86  TCACC                GGTGA(8/7)
MaeII          62  ACGT                 A^CGT
MaeIII         85  GTCAC                ^GT[ACGT]AC
MboI          139  GATC                 ^GATC
MslI           46  CACAGGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         139  GATC                 ^GATC
NlaIII        148  CATG                 CATG^
NlaIV         131  GGAGCC               GG[ACGT]^[ACGT]CC
Sau3AI        139  GATC                 ^GATC
SspI          175  AATATT               AAT^ATT
Tsp45I         85  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

HinfI         160  GATTC                G^A[ACGT]TC
              198  GAATC               
MnlI          115  GAGG                 CCTC(7/6)
              210  GAGG                
MwoI            3  GCTGTTCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               68  GCTTCTTGGGC         
SfcI           16  CTACAG               C^T[GA][CT]AG
              165  CTATAG              
TfiI          160  GATTC                G^A[AT]TC
              198  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

BsmAI