Restriction map for
pFN21AE5574
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccIII AclI AfaI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MluI MluNI
MroI MscI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1190 GACGTC GACGT^C
AccI 1261 GTCTAC GT^[AC][GT]AC
AccII 16 CGCG CG^CG
Alw44I 1237 GTGCAC G^TGCAC
ApaI 208 GGGCCC GGGCC^C
ApaLI 1237 GTGCAC G^TGCAC
AvaI 332 CTCGAG C^[CT]CG[GA]G
BamHI 1168 GGATCC G^GATCC
BanI 912 GGCGCC G^G[CT][GA]CC
BbeI 912 GGCGCC GGCGC^C
BcnI 1288 CCCGG CC^[GC]GG
BglI 238 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 598 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 598 GCTCAGC GC^T[ACGT]AGC
BsaI 573 GAGACC GGTCTC(1/5)
BsaWI 1196 TCCGGT [AT]^CCGG[AT]
BseRI 880 CTCCTC GAGGAG(10/8)
BsmBI 1192 CGTCTC CGTCTC(1/5)
BsoBI 332 CTCGAG C^[CT]CG[GA]G
BspHI 305 TCATGA T^CATGA
BsrBI 954 CCGCTC CCGCTC(-3/-3)
BsrFI 1084 GCCGGC [GA]^CCGG[CT]
BssSI 604 CTCGTG CACGAG(-5/-1)
BstUI 16 CGCG CG^CG
BstXI 258 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1168 GGATCC [GA]^GATC[CT]
CelII 598 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1084 GCCGGC [GA]^CCGG[CT]
EcoNI 287 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1131 ATGCAT ATGCA^T
HgaI 1248 GACGC GACGC(5/10)
KasI 912 GGCGCC G^GCGCC
MfeI 535 CAATTG C^AATTG
MflI 1168 GGATCC [GA]^GATC[CT]
MslI 838 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 535 CAATTG C^AATTG
MvnI 16 CGCG CG^CG
NaeI 1084 GCCGGC GCC^GGC
NarI 912 GGCGCC GG^CGCC
NciI 1288 CCCGG CC^[GC]GG
NgoMIV 1084 GCCGGC G^CCGGC
NsiI 1131 ATGCAT ATGCA^T
PaeR7I 332 CTCGAG C^TCGAG
PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1186 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 305 TCATGA T^CATGA
SfoI 912 GGCGCC GGC^GCC
SmlI 332 CTCGAG C^T[CT][GA]AG
XcmI 1128 CCAATGCATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 332 CTCGAG C^TCGAG
XhoII 1168 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 912 GGCGCC G[GA]^CG[CT]C
1190 GACGTC
BsaHI 912 GGCGCC G[GA]^CG[CT]C
1190 GACGTC
BstF5I 1318 CATCC GGATG(2/0)
843 GGATG
DraII 61 AGGTCCC [GA]G^G[ACGT]CC[CT]
620 GGGGCCT
DsaI 482 CCACGG C^C[GA][CT]GG
1172 CCGTGG
EcoO109I 61 AGGTCCC [GA]G^G[ACGT]CC[CT]
620 GGGGCCT
FauI 902 CCCGC CCCGC(4/6)
562 GCGGG
FokI 1318 CATCC GGATG(9/13)
843 GGATG
HaeII 477 AGCGCC [GA]GCGC^[CT]
912 GGCGCC
Hin1I 912 GGCGCC G[GA]^CG[CT]C
1190 GACGTC
MaeII 734 ACGT A^CGT
1191 ACGT
MseI 425 TTAA T^TAA
1358 TTAA
MspA1I 184 CAGCTG C[AC]G^C[GT]G
263 CAGCTG
NlaIII 306 CATG CATG^
1056 CATG
PleI 4 GAGTC GAGTC(4/5)
1275 GAGTC
PvuII 184 CAGCTG CAG^CTG
263 CAGCTG
SacI 805 GAGCTC GAGCT^C
963 GAGCTC
Tru9I 425 TTAA T^TAA
1358 TTAA
Tsp45I 1161 GTCAC ^GT[GC]AC
1277 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanII BbvI BcgI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp509I TspRI TthHB8I