Restriction map for
pFN21AE6560
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaI BseAI BseRI BsiHKAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI Ksp632I KspI MamI
MboII MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 194 GGTACC G^GTACC
AccII 191 CGCG CG^CG
AcyI 34 GGCGCC G[GA]^CG[CT]C
AgeI 87 ACCGGT A^CCGGT
AlwNI 133 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 356 GGGCCC GGGCC^C
Asp718I 194 GGTACC G^GTACC
BbeI 34 GGCGCC GGCGC^C
BbvCI 472 GCTGAGG CCTCAGC(-5/-2)
BcgI 180 CGAAGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 93 CCCGG CC^[GC]GG
BfaI 230 CTAG C^TAG
BsaAI 169 CACGTA [CT]AC^GT[GA]
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsaWI 87 ACCGGT [AT]^CCGG[AT]
BsgI 220 GTGCAG GTGCAG(16/14)
BsiEI 85 CGACCG CG[GA][CT]^CG
BsiWI 171 CGTACG C^GTACG
BsmAI 14 GAGAC GTCTC(1/5)
BspMI 348 GCAGGT ACCTGC(4/8)
BsrFI 87 ACCGGT [GA]^CCGG[CT]
BsrI 484 CCAGT ACTGG(1/-1)
BssSI 9 CACGAG CACGAG(-5/-1)
BstUI 191 CGCG CG^CG
BstYI 404 AGATCC [GA]^GATC[CT]
Cfr10I 87 ACCGGT [GA]^CCGG[CT]
DsaI 56 CCATGG C^C[GA][CT]GG
EcoNI 468 CCTTGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 56 CCATGG C^C[AT][AT]GG
HaeII 34 GGCGCC [GA]GCGC^[CT]
HgaI 397 GACGC GACGC(5/10)
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HphI 351 GGTGA GGTGA(8/7)
KasI 34 GGCGCC G^GCGCC
KpnI 194 GGTACC GGTAC^C
MaeI 230 CTAG C^TAG
MflI 404 AGATCC [GA]^GATC[CT]
MvnI 191 CGCG CG^CG
NarI 34 GGCGCC GG^CGCC
NciI 93 CCCGG CC^[GC]GG
NcoI 56 CCATGG C^CATGG
PaeR7I 154 CTCGAG C^TCGAG
PinAI 87 ACCGGT A^CCGGT
PpuMI 308 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 160 CTGCAG CTGCA^G
SfaNI 416 GCATC GCATC(5/9)
SfcI 160 CTGCAG C^T[GA][CT]AG
SfoI 34 GGCGCC GGC^GCC
SmlI 154 CTCGAG C^T[CT][GA]AG
StuI 465 AGGCCT AGG^CCT
StyI 56 CCATGG C^C[AT][AT]GG
TaqI 155 TCGA T^CGA
TthHB8I 155 TCGA T^CGA
XcmI 47 CCATCCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 154 CTCGAG C^TCGAG
XhoII 404 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AluI 158 AGCT AG^CT
333 AGCT
AlwI 405 GATCC GGATC(4/5)
429 GATCC
AocI 282 CCTGAGG CC^T[ACGT]AGG
434 CCTTAGG
AvaI 154 CTCGAG C^[CT]CG[GA]G
359 CCCGAG
BanI 34 GGCGCC G^G[CT][GA]CC
194 GGTACC
BanII 4 GAGCCC G[GA]GC[CT]^C
356 GGGCCC
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
356 GGGCCC
BsaJI 56 CCATGG C^C[ACGT][ACGT]GG
305 CCTGGG
BsmFI 395 GGGAC GGGAC(10/14)
91 GTCCC
BsoBI 154 CTCGAG C^[CT]CG[GA]G
359 CCCGAG
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
356 GGGCCC
Bsu36I 282 CCTGAGG CC^T[ACGT]AGG
434 CCTTAGG
CfoI 35 GCGC GCG^C
190 GCGC
DpnII 405 GATC ^GATC
429 GATC
DraII 308 GGGTCCT [GA]G^G[ACGT]CC[CT]
355 AGGGCCC
Eco81I 282 CCTGAGG CC^T[ACGT]AGG
434 CCTTAGG
EcoO109I 308 GGGTCCT [GA]G^G[ACGT]CC[CT]
355 AGGGCCC
FauI 21 CCCGC CCCGC(4/6)
392 GCGGG
HapII 88 CCGG C^CGG
94 CCGG
HhaI 35 GCGC GCG^C
190 GCGC
HinP1I 35 GCGC G^CGC
190 GCGC
HinfI 247 GAGTC G^A[ACGT]TC
423 GACTC
HpaII 88 CCGG C^CGG
94 CCGG
MaeIII 124 GTGAC ^GT[ACGT]AC
452 GTGAC
MboI 405 GATC ^GATC
429 GATC
MspA1I 22 CCGCTG C[AC]G^C[GT]G
291 CCGCTG
MspI 88 CCGG C^CGG
94 CCGG
MwoI 37 GCCTTTTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
472 GCTGAGGCTGC
NdeII 405 GATC ^GATC
429 GATC
NlaIII 57 CATG CATG^
241 CATG
PleI 423 GACTC GAGTC(4/5)
247 GAGTC
Sau3AI 405 GATC ^GATC
429 GATC
Tsp45I 124 GTGAC ^GT[GC]AC
452 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AfaI AvaII BbvI BsiYI
BslI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI FokI
HaeIII ItaI MaeII MnlI MvaI
NlaIV RsaI Sau96I ScrFI TseI