##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MroI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI          345  GCGG                 CCGC(-3/-1)
AspHI          53  GAGCTC               G[AT]GC[AT]^C
AvaII         187  GGACC                G^G[AT]CC
BalI          294  TGGCCA               TGG^CCA
BfaI          508  CTAG                 C^TAG
BpmI          259  CTGGAG               CTGGAG(16/14)
BsiHKAI        53  GAGCTC               G[AT]GC[AT]^C
BsmAI         154  GAGAC                GTCTC(1/5)
BssKI          68  CCTGG                ^CC[ACGT]GG
BstF5I         65  CATCC                GGATG(2/0)
BstNI          68  CCTGG                CC^[AT]GG
Cfr13I        187  GGACC                G^G[ACGT]CC
DpnII          62  GATC                 ^GATC
EaeI          294  TGGCCA               [CT]^GGCC[GA]
EcoRII         68  CCTGG                ^CC[AT]GG
Fnu4HI        345  GCGGC                GC^[ACGT]GC
FokI           65  CATCC                GGATG(9/13)
HaeIII        295  GGCC                 GG^CC
ItaI          345  GCGGC                GC^[ACGT]GC
MaeI          508  CTAG                 C^TAG
MboI           62  GATC                 ^GATC
MboII         217  TCTTC                GAAGA(8/7)
MluNI         294  TGGCCA               TGG^CCA
MscI          294  TGGCCA               TGG^CCA
MvaI           68  CCTGG                CC^[AT]GG
MwoI          304  GCTCCCAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          62  GATC                 ^GATC
NspI          426  GCATGT               [GA]CATG^[CT]
SacI           53  GAGCTC               GAGCT^C
Sau3AI         62  GATC                 ^GATC
Sau96I        187  GGACC                G^G[ACGT]CC
ScrFI          68  CCTGG                CC^[ACGT]GG
SmlI           47  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BanII          53  GAGCTC               G[GA]GC[CT]^C
              166  GAGCCC              
BmyI           53  GAGCTC               G[AGT]GC[ACT]^C
              166  GAGCCC              
BsiYI         119  CCTTCAGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              197  CCTCAAAATGG         
BslI          119  CCTTCAGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              197  CCTCAAAATGG         
BsmFI         412  GGGAC                GGGAC(10/14)
              434  GGGAC               
Bsp1286I       53  GAGCTC               G[AGT]GC[ACT]^C
              166  GAGCCC              
BsrI          251  ACTGG                ACTGG(1/-1)
              491  ACTGG               
MseI           31  TTAA                 T^TAA
              286  TTAA                
NlaIII        366  CATG                 CATG^
              427  CATG                
NlaIV         165  GGAGCC               GG[ACGT]^[ACGT]CC
              303  GGCTCC              
SfaNI         214  GCATC                GCATC(5/9)
              465  GCATC               
Tru9I          31  TTAA                 T^TAA
              286  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        DdeI        MnlI        Tsp509I