Restriction map for
pFN21AE5544
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NarI NcoI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1042 GTCTAC GT^[AC][GT]AC
AcsI 917 AAATTC [GA]^AATT[CT]
AflII 1054 CTTAAG C^TTAAG
AflIII 331 ACATGT A^C[GA][CT]GT
AocI 16 CCTAAGG CC^T[ACGT]AGG
ApoI 917 AAATTC [GA]^AATT[CT]
AspHI 490 GAGCAC G[AT]GC[AT]^C
BfaI 133 CTAG C^TAG
BfrI 1054 CTTAAG C^TTAAG
BsaAI 1032 TACGTG [CT]AC^GT[GA]
BsiEI 976 CGGCCG CG[GA][CT]^CG
BsiHKAI 490 GAGCAC G[AT]GC[AT]^C
BsmBI 238 GAGACG CGTCTC(1/5)
Bsp1407I 644 TGTACA T^GTACA
BspLU11I 331 ACATGT A^CATGT
BsrFI 974 GCCGGC [GA]^CCGG[CT]
BsrGI 644 TGTACA T^GTACA
BstAPI 771 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 16 CCTAAGG CC^T[ACGT]AGG
CfoI 244 GCGC GCG^C
Cfr10I 974 GCCGGC [GA]^CCGG[CT]
EagI 976 CGGCCG C^GGCCG
EarI 123 GAAGAG CTCTTC(1/4)
EclXI 976 CGGCCG C^GGCCG
Eco52I 976 CGGCCG C^GGCCG
Eco81I 16 CCTAAGG CC^T[ACGT]AGG
FseI 973 GGCCGGCC GGCCGG^CC
HaeII 243 GGCGCT [GA]GCGC^[CT]
HgaI 1040 GCGTC GACGC(5/10)
HhaI 244 GCGC GCG^C
HinP1I 244 GCGC G^CGC
HindIII 103 AAGCTT A^AGCTT
Ksp632I 123 GAAGAG CTCTTC(1/4)
MaeI 133 CTAG C^TAG
MboII 123 GAAGA GAAGA(8/7)
MseI 1055 TTAA T^TAA
MslI 756 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 974 GCCGGC GCC^GGC
NdeI 109 CATATG CA^TATG
NgoMIV 974 GCCGGC G^CCGGC
NspI 331 ACATGT [GA]CATG^[CT]
PaeR7I 1085 CTCGAG C^TCGAG
SexAI 392 ACCTGGT A^CC[AT]GGT
SmaI 31 CCCGGG CCC^GGG
SspBI 644 TGTACA T^GTACA
Tru9I 1055 TTAA T^TAA
Tsp45I 209 GTGAC ^GT[GC]AC
Tsp509I 918 AATT ^AATT
XbaI 132 TCTAGA T^CTAGA
XcmI 798 CCAGTGGACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1085 CTCGAG C^TCGAG
XmaCI 31 CCCGGG C^CCGGG
XmaI 31 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 28 GGGCCC GGGCC^C
569 GGGCCC
AvaI 31 CCCGGG C^[CT]CG[GA]G
1085 CTCGAG
BcnI 31 CCCGG CC^[GC]GG
32 CCGGG
BsaI 718 GGTCTC GGTCTC(1/5)
888 GGTCTC
BsoBI 31 CCCGGG C^[CT]CG[GA]G
1085 CTCGAG
DraII 27 AGGGCCC [GA]G^G[ACGT]CC[CT]
568 AGGGCCC
EaeI 972 TGGCCG [CT]^GGCC[GA]
976 CGGCCG
EcoO109I 27 AGGGCCC [GA]G^G[ACGT]CC[CT]
568 AGGGCCC
EcoT14I 158 CCAAGG C^C[AT][AT]GG
284 CCTTGG
FauI 413 CCCGC CCCGC(4/6)
467 CCCGC
HapII 32 CCGG C^CGG
975 CCGG
HpaII 32 CCGG C^CGG
975 CCGG
MaeIII 209 GTGAC ^GT[ACGT]AC
752 GTAAC
MspI 32 CCGG C^CGG
975 CCGG
NciI 31 CCCGG CC^[GC]GG
32 CCGGG
PflMI 90 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAAATTCTGG
PstI 115 CTGCAG CTGCA^G
685 CTGCAG
SmlI 1054 CTTAAG C^T[CT][GA]AG
1085 CTCGAG
StyI 158 CCAAGG C^C[AT][AT]GG
284 CCTTGG
TfiI 129 GATTC G^A[AT]TC
1125 GATTC
Van91I 90 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAAATTCTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I