Restriction map for
pFN21AE9685
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT14I FauI
FbaI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 884 GACGCC G[GA]^CG[CT]C
AflIII 192 ACATGT A^C[GA][CT]GT
AlwNI 272 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BglI 518 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 884 GACGCC G[GA]^CG[CT]C
BseRI 620 GAGGAG GAGGAG(10/8)
BsgI 419 CTGCAC GTGCAG(16/14)
BsmAI 589 GTCTC GTCTC(1/5)
BsmFI 724 GGGAC GGGAC(10/14)
BsmI 565 GAATGC GAATGC(1/-1)
BspHI 206 TCATGA T^CATGA
BspLU11I 192 ACATGT A^CATGT
BsrDI 50 CATTGC GCAATG(2/0)
BsrI 231 ACTGG ACTGG(1/-1)
BssSI 38 CTCGTG CACGAG(-5/-1)
Cac8I 745 GCAAGC GC[ACGT]^[ACGT]GC
DraI 226 TTTAAA TTT^AAA
DraII 330 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 330 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 202 GATATC GAT^ATC
EcoT22I 47 ATGCAT ATGCA^T
HaeIII 517 GGCC GG^CC
Hin1I 884 GACGCC G[GA]^CG[CT]C
HindIII 634 AAGCTT A^AGCTT
MaeIII 362 GTGAC ^GT[ACGT]AC
NsiI 47 ATGCAT ATGCA^T
NspI 192 ACATGT [GA]CATG^[CT]
PpuMI 330 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 206 TCATGA T^CATGA
ScaI 504 AGTACT AGT^ACT
SfaNI 924 GATGC GCATC(5/9)
SmlI 889 CTTGAG C^T[CT][GA]AG
TaqI 663 TCGA T^CGA
Tsp45I 362 GTGAC ^GT[GC]AC
TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 663 TCGA T^CGA
XcmI 821 CCAAAATGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 253 GCGG CCGC(-3/-1)
763 GCGG
AvaII 293 GGTCC G^G[AT]CC
331 GGACC
BbvI 841 GCAGC GCAGC(8/12)
768 GCTGC
BfaI 217 CTAG C^TAG
335 CTAG
BpmI 142 CTCCAG CTGGAG(16/14)
674 CTCCAG
BsiYI 322 CCCTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
523 CCTGGCACGGG
BslI 322 CCCTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
523 CCTGGCACGGG
BstF5I 783 GGATG GGATG(2/0)
923 GGATG
EarI 591 CTCTTC CTCTTC(1/4)
488 GAAGAG
FokI 783 GGATG GGATG(9/13)
923 GGATG
Ksp632I 591 CTCTTC CTCTTC(1/4)
488 GAAGAG
MaeI 217 CTAG C^TAG
335 CTAG
MwoI 147 GCAGAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
518 GCCCACCTGGC
SfcI 560 CTACAG C^T[GA][CT]AG
711 CTGTAG
TfiI 625 GAATC G^A[AT]TC
899 GAATC
TseI 768 GCTGC G^C[AT]GC
841 GCAGC
Tsp509I 32 AATT ^AATT
850 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI BbsI BpuAI BsaJI
BssKI BstNI Cfr13I DdeI EcoRII
Fnu4HI HgaI HinfI HphI ItaI
MboII MnlI MseI MvaI NlaIII
PleI RsaI Sau96I ScrFI Tru9I