################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 884 GACGCC G[GA]^CG[CT]C AflIII 192 ACATGT A^C[GA][CT]GT AlwNI 272 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG BglI 518 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 884 GACGCC G[GA]^CG[CT]C BseRI 620 GAGGAG GAGGAG(10/8) BsgI 419 CTGCAC GTGCAG(16/14) BsmAI 589 GTCTC GTCTC(1/5) BsmFI 724 GGGAC GGGAC(10/14) BsmI 565 GAATGC GAATGC(1/-1) BspHI 206 TCATGA T^CATGA BspLU11I 192 ACATGT A^CATGT BsrDI 50 CATTGC GCAATG(2/0) BsrI 231 ACTGG ACTGG(1/-1) BssSI 38 CTCGTG CACGAG(-5/-1) Cac8I 745 GCAAGC GC[ACGT]^[ACGT]GC DraI 226 TTTAAA TTT^AAA DraII 330 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 330 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 202 GATATC GAT^ATC EcoT22I 47 ATGCAT ATGCA^T HaeIII 517 GGCC GG^CC Hin1I 884 GACGCC G[GA]^CG[CT]C HindIII 634 AAGCTT A^AGCTT MaeIII 362 GTGAC ^GT[ACGT]AC NsiI 47 ATGCAT ATGCA^T NspI 192 ACATGT [GA]CATG^[CT] PpuMI 330 AGGACCT [GA]G^G[AT]CC[CT] RcaI 206 TCATGA T^CATGA ScaI 504 AGTACT AGT^ACT SfaNI 924 GATGC GCATC(5/9) SmlI 889 CTTGAG C^T[CT][GA]AG TaqI 663 TCGA T^CGA Tsp45I 362 GTGAC ^GT[GC]AC TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 663 TCGA T^CGA XcmI 821 CCAAAATGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 253 GCGG CCGC(-3/-1) 763 GCGG AvaII 293 GGTCC G^G[AT]CC 331 GGACC BbvI 841 GCAGC GCAGC(8/12) 768 GCTGC BfaI 217 CTAG C^TAG 335 CTAG BpmI 142 CTCCAG CTGGAG(16/14) 674 CTCCAG BsiYI 322 CCCTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 523 CCTGGCACGGG BslI 322 CCCTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 523 CCTGGCACGGG BstF5I 783 GGATG GGATG(2/0) 923 GGATG EarI 591 CTCTTC CTCTTC(1/4) 488 GAAGAG FokI 783 GGATG GGATG(9/13) 923 GGATG Ksp632I 591 CTCTTC CTCTTC(1/4) 488 GAAGAG MaeI 217 CTAG C^TAG 335 CTAG MwoI 147 GCAGAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 518 GCCCACCTGGC SfcI 560 CTACAG C^T[GA][CT]AG 711 CTGTAG TfiI 625 GAATC G^A[AT]TC 899 GAATC TseI 768 GCTGC G^C[AT]GC 841 GCAGC Tsp509I 32 AATT ^AATT 850 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI BbsI BpuAI BsaJI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HgaI HinfI HphI ItaI MboII MnlI MseI MvaI NlaIII PleI RsaI Sau96I ScrFI Tru9I