Restriction map for
pFN21AE5641
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AvaI AviII AvrII
BanII BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 1537 GGTACC G^GTACC
AcyI 3 GGCGCC G[GA]^CG[CT]C
Asp718I 1537 GGTACC G^GTACC
BamHI 1238 GGATCC G^GATCC
BbeI 3 GGCGCC GGCGC^C
BbvCI 1394 CCTCAGC CCTCAGC(-5/-2)
BciVI 1711 GTATCC GTATCC(6/5)
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsgI 1517 GTGCAG GTGCAG(16/14)
BsmI 1077 GAATGC GAATGC(1/-1)
BstAPI 639 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1347 GGTGACC G^GT[ACGT]ACC
BstPI 1347 GGTGACC G^GT[ACGT]ACC
BstXI 1049 CCAGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4 GCGC GCG^C
DrdI 1378 GACTATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1736 CCGTGG C^C[GA][CT]GG
EcoO65I 1347 GGTGACC G^GT[ACGT]ACC
HaeII 3 GGCGCC [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HinP1I 4 GCGC G^CGC
KasI 3 GGCGCC G^GCGCC
KpnI 1537 GGTACC GGTAC^C
MaeII 683 ACGT A^CGT
NarI 3 GGCGCC GG^CGCC
NdeI 546 CATATG CA^TATG
PshAI 895 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1570 CAGCTG CAG^CTG
SfcI 171 CTACAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
SspI 615 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 223 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
892 GAAGACATTC
AspHI 142 GTGCTC G[AT]GC[AT]^C
1466 GAGCAC
AspI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1605 GACGGTGTC
BbsI 892 GAAGAC GAAGAC(2/6)
659 GTCTTC
BbvI 639 GCAGC GCAGC(8/12)
1399 GCAGC
BcnI 18 CCCGG CC^[GC]GG
600 CCGGG
BpuAI 892 GAAGAC GAAGAC(2/6)
659 GTCTTC
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
1466 GAGCAC
BsmAI 578 GTCTC GTCTC(1/5)
939 GTCTC
BspHI 216 TCATGA T^CATGA
1166 TCATGA
BspMI 488 ACCTGC ACCTGC(4/8)
1727 ACCTGC
BsrDI 294 CATTGC GCAATG(2/0)
1510 CATTGC
EarI 227 CTCTTC CTCTTC(1/4)
52 GAAGAG
FauI 90 CCCGC CCCGC(4/6)
1175 CCCGC
Ksp632I 227 CTCTTC CTCTTC(1/4)
52 GAAGAG
MslI 542 CACCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1510 CATTGCAGTG
MspA1I 91 CCGCTG C[AC]G^C[GT]G
1570 CAGCTG
NciI 18 CCCGG CC^[GC]GG
600 CCGGG
PflFI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1605 GACGGTGTC
RcaI 216 TCATGA T^CATGA
1166 TCATGA
ScaI 1211 AGTACT AGT^ACT
1658 AGTACT
SmlI 1365 CTTGAG C^T[CT][GA]AG
1419 CTTGAG
TseI 639 GCAGC G^C[AT]GC
1399 GCAGC
Tth111I 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1605 GACGGTGTC
XcmI 830 CCATCCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1265 CCAATCTAGGCGTGG
XmnI 223 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
892 GAAGACATTC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BalI
BanI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII