Restriction map for
pFN21AE5554
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI StuI StyI
SwaI Tru9I Tth111I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 917 GAATTC [GA]^AATT[CT]
AfaI 432 GTAC GT^AC
AhdI 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 940 CCTGAGG CC^T[ACGT]AGG
ApoI 917 GAATTC [GA]^AATT[CT]
AspEI 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 130 GAGCTC G[AT]GC[AT]^C
BbsI 331 GAAGAC GAAGAC(2/6)
BfaI 733 CTAG C^TAG
BpuAI 331 GAAGAC GAAGAC(2/6)
BsaI 295 GAGACC GGTCTC(1/5)
BseRI 1049 GAGGAG GAGGAG(10/8)
BsgI 790 GTGCAG GTGCAG(16/14)
BsiHKAI 130 GAGCTC G[AT]GC[AT]^C
BsmBI 783 GAGACG CGTCTC(1/5)
BsmI 109 GAATGC GAATGC(1/-1)
Bsp1407I 431 TGTACA T^GTACA
BsrBI 769 GAGCGG CCGCTC(-3/-3)
BsrGI 431 TGTACA T^GTACA
BssSI 1258 CACGAG CACGAG(-5/-1)
Bsu36I 940 CCTGAGG CC^T[ACGT]AGG
DsaI 822 CCACGG C^C[GA][CT]GG
Eam1105I 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 940 CCTGAGG CC^T[ACGT]AGG
EcoRI 917 GAATTC G^AATTC
HindIII 899 AAGCTT A^AGCTT
MaeI 733 CTAG C^TAG
NaeI 889 GCCGGC GCC^GGC
NgoMIV 889 GCCGGC G^CCGGC
NspI 276 ACATGC [GA]CATG^[CT]
RsaI 432 GTAC GT^AC
SacI 130 GAGCTC GAGCT^C
SbfI 54 CCTGCAGG CCTGCA^GG
SmaI 21 CCCGGG CCC^GGG
Sse8387I 54 CCTGCAGG CCTGCA^GG
SspBI 431 TGTACA T^GTACA
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 826 GGCGTC G[GA]^CG[CT]C
991 GACGCC
AlwI 214 GATCC GGATC(4/5)
739 GATCC
BanI 449 GGCACC G^G[CT][GA]CC
702 GGCACC
BcgI 225 CGAGGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
837 CGAGGGCCCTGC
BpmI 657 CTCCAG CTGGAG(16/14)
1064 CTGGAG
BsaHI 826 GGCGTC G[GA]^CG[CT]C
991 GACGCC
BspMI 57 GCAGGT ACCTGC(4/8)
1090 GCAGGT
BsrDI 597 CATTGC GCAATG(2/0)
806 GCAATG
BsrFI 676 GCCGGT [GA]^CCGG[CT]
889 GCCGGC
Cfr10I 676 GCCGGT [GA]^CCGG[CT]
889 GCCGGC
EarI 441 GAAGAG CTCTTC(1/4)
515 GAAGAG
FauI 1102 CCCGC CCCGC(4/6)
206 GCGGG
Hin1I 826 GGCGTC G[GA]^CG[CT]C
991 GACGCC
Ksp632I 441 GAAGAG CTCTTC(1/4)
515 GAAGAG
MaeIII 1124 GTCAC ^GT[ACGT]AC
1214 GTCAC
MslI 759 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
802 CACAGCAATG
PaeR7I 985 CTCGAG C^TCGAG
1133 CTCGAG
PpuMI 228 GGGTCCT [GA]G^G[AT]CC[CT]
727 GGGACCC
SapI 441 GAAGAGC GCTCTTC(1/4)
515 GAAGAGC
SexAI 193 ACCTGGT A^CC[AT]GGT
705 ACCAGGT
SmlI 985 CTCGAG C^T[CT][GA]AG
1133 CTCGAG
Tsp45I 1124 GTCAC ^GT[GC]AC
1214 GTCAC
TspRI 392 CACTG CA[GC]TG[ACGT][ACGT]^
882 CACTG
XcmI 191 CCACCTGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
849 CCACATCCCCCCTGG
XhoI 985 CTCGAG C^TCGAG
1133 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DraIII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PshAI PstI PvuII Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI Tsp509I TthHB8I Van91I