################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspMI BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 403 GTATAC GT^[AC][GT]AC AfaI 63 GTAC GT^AC AlwNI 1154 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2206 GGGCCC GGGCC^C AspHI 1210 GAGCAC G[AT]GC[AT]^C BamHI 960 GGATCC G^GATCC BanII 2206 GGGCCC G[GA]GC[CT]^C BciVI 1893 GGATAC GTATCC(6/5) BpmI 422 CTCCAG CTGGAG(16/14) BsaAI 406 TACGTG [CT]AC^GT[GA] BsiHKAI 1210 GAGCAC G[AT]GC[AT]^C BspHI 1749 TCATGA T^CATGA BspLU11I 633 ACATGT A^CATGT BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 201 CATTGC GCAATG(2/0) BsrFI 100 GCCGGC [GA]^CCGG[CT] Bst1107I 403 GTATAC GTA^TAC BstZ17I 403 GTATAC GTA^TAC Cfr10I 100 GCCGGC [GA]^CCGG[CT] EaeI 1014 TGGCCG [CT]^GGCC[GA] MaeII 407 ACGT A^CGT MaeIII 83 GTGAC ^GT[ACGT]AC MspA1I 1434 CAGCTG C[AC]G^C[GT]G NaeI 100 GCCGGC GCC^GGC NdeI 1898 CATATG CA^TATG NgoMIV 100 GCCGGC G^CCGGC NspI 633 ACATGT [GA]CATG^[CT] PaeR7I 1708 CTCGAG C^TCGAG PpuMI 155 GGGACCT [GA]G^G[AT]CC[CT] PstI 287 CTGCAG CTGCA^G PvuII 1434 CAGCTG CAG^CTG RcaI 1749 TCATGA T^CATGA RsaI 63 GTAC GT^AC StuI 1140 AGGCCT AGG^CCT Tsp45I 83 GTGAC ^GT[GC]AC XbaI 1189 TCTAGA T^CTAGA XcmI 1032 CCAGGCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1708 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 407 ACGTGT A^C[GA][CT]GT 633 ACATGT AocI 1126 CCTGAGG CC^T[ACGT]AGG 1143 CCTTAGG Asp700I 865 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1753 GAAAATGTTC AvaI 121 CTCGGG C^[CT]CG[GA]G 1708 CTCGAG AvaII 156 GGACC G^G[AT]CC 766 GGACC BanI 7 GGCACC G^G[CT][GA]CC 926 GGCACC BcnI 315 CCCGG CC^[GC]GG 773 CCCGG BmyI 1210 GAGCAC G[AGT]GC[ACT]^C 2206 GGGCCC BsaI 747 GAGACC GGTCTC(1/5) 1705 GGTCTC BsaJI 314 CCCCGG C^C[ACGT][ACGT]GG 1031 CCCAGG BsmBI 68 GAGACG CGTCTC(1/5) 545 GAGACG BsmI 50 GAATGC GAATGC(1/-1) 1233 GAATGC BsoBI 121 CTCGGG C^[CT]CG[GA]G 1708 CTCGAG Bsp1286I 1210 GAGCAC G[AGT]GC[ACT]^C 2206 GGGCCC BstBI 885 TTCGAA TT^CGAA 2089 TTCGAA BstNI 159 CCTGG CC^[AT]GG 1032 CCAGG Bsu36I 1126 CCTGAGG CC^T[ACGT]AGG 1143 CCTTAGG Cac8I 100 GCCGGC GC[ACGT]^[ACGT]GC 759 GCGAGC CfoI 242 GCGC GCG^C 780 GCGC Eco81I 1126 CCTGAGG CC^T[ACGT]AGG 1143 CCTTAGG EcoNI 1548 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1572 CCTCAACAAGG EcoRII 159 CCTGG ^CC[AT]GG 1032 CCAGG HhaI 242 GCGC GCG^C 780 GCGC HinP1I 242 GCGC G^CGC 780 GCGC HphI 82 GGTGA GGTGA(8/7) 1996 GGTGA MvaI 159 CCTGG CC^[AT]GG 1032 CCAGG NciI 315 CCCGG CC^[GC]GG 773 CCCGG NspV 885 TTCGAA TT^CGAA 2089 TTCGAA SfuI 885 TTCGAA TT^CGAA 2089 TTCGAA XmnI 865 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1753 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstYI Cfr13I DdeI DpnII DraII EarI EcoO109I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MslI MspI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII