Restriction map for
pFN21AE5761
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BamHI BbeI
BbrPI BcgI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Aor51HI 2186 AGCGCT AGC^GCT
AspI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 456 GGTGCC G^G[CT][GA]CC
BbvCI 1202 CCTCAGC CCTCAGC(-5/-2)
BclI 372 TGATCA T^GATCA
BglI 2267 GCCGGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 1719 CGATCG CG[GA][CT]^CG
Bsp1407I 1389 TGTACA T^GTACA
BsrGI 1389 TGTACA T^GTACA
BstAPI 2373 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1275 AGATCC [GA]^GATC[CT]
DraII 182 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 602 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 323 TGGCCG [CT]^GGCC[GA]
Eco47III 2186 AGCGCT AGC^GCT
EcoO109I 182 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 2392 CCCGC CCCGC(4/6)
FbaI 372 TGATCA T^GATCA
HaeII 2186 AGCGCT [GA]GCGC^[CT]
MaeII 2258 ACGT A^CGT
MfeI 1238 CAATTG C^AATTG
MflI 1275 AGATCC [GA]^GATC[CT]
MslI 1542 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1238 CAATTG C^AATTG
PaeR7I 1193 CTCGAG C^TCGAG
PflFI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 182 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2050 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1054 CTGCAG CTGCA^G
PvuI 1719 CGATCG CGAT^CG
SacI 228 GAGCTC GAGCT^C
SapI 589 GAAGAGC GCTCTTC(1/4)
SbfI 1053 CCTGCAGG CCTGCA^GG
Sse8387I 1053 CCTGCAGG CCTGCA^GG
SspBI 1389 TGTACA T^GTACA
Tth111I 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1410 CCAGCCTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1193 CTCGAG C^TCGAG
XhoII 1275 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 560 GATCC GGATC(4/5)
1276 GATCC
AvaII 183 GGACC G^G[AT]CC
1255 GGACC
AviII 284 TGCGCA TGC^GCA
1292 TGCGCA
BanII 228 GAGCTC G[GA]GC[CT]^C
1305 GAGCCC
BbsI 676 GAAGAC GAAGAC(2/6)
1770 GTCTTC
BciVI 261 GGATAC GTATCC(6/5)
1662 GGATAC
BpuAI 676 GAAGAC GAAGAC(2/6)
1770 GTCTTC
BsaI 901 GAGACC GGTCTC(1/5)
1083 GAGACC
BsrDI 311 GCAATG GCAATG(2/0)
2164 GCAATG
BsrFI 269 ACCGGC [GA]^CCGG[CT]
2267 GCCGGT
Cfr10I 269 ACCGGC [GA]^CCGG[CT]
2267 GCCGGT
EcoNI 351 CCTTGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
447 CCTTGATAAGG
EcoRI 867 GAATTC G^AATTC
1509 GAATTC
FspI 284 TGCGCA TGC^GCA
1292 TGCGCA
HapII 270 CCGG C^CGG
2268 CCGG
HpaII 270 CCGG C^CGG
2268 CCGG
MspI 270 CCGG C^CGG
2268 CCGG
PflMI 1624 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2206 CCAGGCTCTGG
PleI 1726 GACTC GAGTC(4/5)
2055 GAGTC
Van91I 1624 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2206 CCAGGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I