Restriction map for
pFN21AE5651
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 25 CGCG CG^CG
AcsI 1031 GAATTC [GA]^AATT[CT]
AflIII 1407 ACATGT A^C[GA][CT]GT
ApaI 335 GGGCCC GGGCC^C
ApoI 1031 GAATTC [GA]^AATT[CT]
AvaII 370 GGACC G^G[AT]CC
BalI 1226 TGGCCA TGG^CCA
BclI 1499 TGATCA T^GATCA
BlpI 304 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 304 GCTGAGC GC^T[ACGT]AGC
BsaBI 1010 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1339 GTGCAG GTGCAG(16/14)
BsmBI 427 CGTCTC CGTCTC(1/5)
BsmI 242 GCATTC GAATGC(1/-1)
BspLU11I 1407 ACATGT A^CATGT
BsrBI 331 GAGCGG CCGCTC(-3/-3)
BsrDI 1389 GCAATG GCAATG(2/0)
BstUI 25 CGCG CG^CG
CelII 304 GCTGAGC GC^T[ACGT]AGC
CfoI 1414 GCGC GCG^C
EcoRI 1031 GAATTC G^AATTC
FbaI 1499 TGATCA T^GATCA
HhaI 1414 GCGC GCG^C
HinP1I 1414 GCGC G^CGC
HincII 491 GTCAAC GT[CT]^[GA]AC
HindII 491 GTCAAC GT[CT]^[GA]AC
MaeII 1119 ACGT A^CGT
MamI 1010 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1226 TGGCCA TGG^CCA
MscI 1226 TGGCCA TGG^CCA
MvnI 25 CGCG CG^CG
NheI 939 GCTAGC G^CTAGC
NruI 24 TCGCGA TCG^CGA
PaeR7I 1280 CTCGAG C^TCGAG
SacI 1211 GAGCTC GAGCT^C
ScaI 638 AGTACT AGT^ACT
SfiI 327 GGCCGAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1280 CTCGAG C^T[CT][GA]AG
SphI 815 GCATGC GCATG^C
XcmI 1196 CCAAGGTGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1280 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 173 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
646 CAGGGCCTG
AspHI 1180 GTGCTC G[AT]GC[AT]^C
1211 GAGCTC
BamHI 37 GGATCC G^GATCC
520 GGATCC
BcnI 41 CCGGG CC^[GC]GG
671 CCCGG
BglI 328 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1270 GCCCTGGTGGC
BpmI 744 CTCCAG CTGGAG(16/14)
401 CTGGAG
BseRI 257 CTCCTC GAGGAG(10/8)
880 GAGGAG
BsiHKAI 1180 GTGCTC G[AT]GC[AT]^C
1211 GAGCTC
BspMI 1154 ACCTGC ACCTGC(4/8)
702 GCAGGT
BsrFI 1058 GCCGGC [GA]^CCGG[CT]
1088 GCCGGC
Cfr10I 1058 GCCGGC [GA]^CCGG[CT]
1088 GCCGGC
DraII 335 GGGCCCT [GA]G^G[ACGT]CC[CT]
647 AGGGCCT
EarI 535 CTCTTC CTCTTC(1/4)
877 GAAGAG
EcoNI 339 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
837 CCTTAACAAGG
EcoO109I 335 GGGCCCT [GA]G^G[ACGT]CC[CT]
647 AGGGCCT
Ksp632I 535 CTCTTC CTCTTC(1/4)
877 GAAGAG
MseI 476 TTAA T^TAA
839 TTAA
MslI 852 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1132 CATGCTGGTG
MspA1I 275 CAGCTG C[AC]G^C[GT]G
398 CAGCTG
NaeI 1058 GCCGGC GCC^GGC
1088 GCCGGC
NciI 41 CCGGG CC^[GC]GG
671 CCCGG
NcoI 617 CCATGG C^CATGG
1093 CCATGG
NgoMIV 1058 GCCGGC G^CCGGC
1088 GCCGGC
NspI 815 GCATGC [GA]CATG^[CT]
1407 ACATGT
PflMI 172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCAGGGCCTGG
PvuII 275 CAGCTG CAG^CTG
398 CAGCTG
Tru9I 476 TTAA T^TAA
839 TTAA
Tsp509I 954 AATT ^AATT
1032 AATT
Van91I 172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaI BanII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DsaI EaeI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII