Restriction map for
pFN21AE2424
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BfaI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BsiWI BsmI BspLU11I BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 2274 CGCG CG^CG
AccIII 2204 TCCGGA T^CCGGA
AgeI 807 ACCGGT A^CCGGT
AlwNI 2301 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1586 TGCGCA TGC^GCA
BamHI 1616 GGATCC G^GATCC
BbeI 231 GGCGCC GGCGC^C
BbrPI 1999 CACGTG CAC^GTG
BcgI 2351 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2454 GGATAC GTATCC(6/5)
BclI 1394 TGATCA T^GATCA
BglI 1822 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 957 CTGGAG CTGGAG(16/14)
BseAI 2204 TCCGGA T^CCGGA
BsmBI 81 CGTCTC CGTCTC(1/5)
Bsp1407I 1739 TGTACA T^GTACA
BspDI 1237 ATCGAT AT^CGAT
BspEI 2204 TCCGGA T^CCGGA
BspHI 2195 TCATGA T^CATGA
BsrBI 2038 CCGCTC CCGCTC(-3/-3)
BsrGI 1739 TGTACA T^GTACA
BstAPI 2243 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2274 CGCG CG^CG
ClaI 1237 ATCGAT AT^CGAT
DrdI 1246 GACAAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2278 CTCTTC CTCTTC(1/4)
FbaI 1394 TGATCA T^GATCA
FspI 1586 TGCGCA TGC^GCA
HgaI 94 GACGC GACGC(5/10)
KasI 231 GGCGCC G^GCGCC
Ksp632I 2278 CTCTTC CTCTTC(1/4)
MroI 2204 TCCGGA T^CCGGA
MvnI 2274 CGCG CG^CG
NarI 231 GGCGCC GG^CGCC
NcoI 1646 CCATGG C^CATGG
PaeR7I 1034 CTCGAG C^TCGAG
PflFI 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 807 ACCGGT A^CCGGT
PmaCI 1999 CACGTG CAC^GTG
PmlI 1999 CACGTG CAC^GTG
PstI 2321 CTGCAG CTGCA^G
PvuII 1387 CAGCTG CAG^CTG
RcaI 2195 TCATGA T^CATGA
SapI 2277 GCTCTTC GCTCTTC(1/4)
ScaI 515 AGTACT AGT^ACT
SfoI 231 GGCGCC GGC^GCC
SphI 1760 GCATGC GCATG^C
SspBI 1739 TGTACA T^GTACA
StuI 578 AGGCCT AGG^CCT
Tth111I 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1193 CCATGAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1034 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 94 GACGCC G[GA]^CG[CT]C
231 GGCGCC
Aor51HI 1012 AGCGCT AGC^GCT
1135 AGCGCT
ApaI 70 GGGCCC GGGCC^C
1577 GGGCCC
BbvCI 1500 CCTCAGC CCTCAGC(-5/-2)
1932 CCTCAGC
BglII 1331 AGATCT A^GATCT
2474 AGATCT
BsaHI 94 GACGCC G[GA]^CG[CT]C
231 GGCGCC
BsaWI 807 ACCGGT [AT]^CCGG[AT]
2204 TCCGGA
BsiEI 44 CGGTCG CG[GA][CT]^CG
809 CGGTCG
BsmAI 82 GTCTC GTCTC(1/5)
852 GTCTC
BspMI 923 ACCTGC ACCTGC(4/8)
2165 ACCTGC
BsrFI 807 ACCGGT [GA]^CCGG[CT]
1958 GCCGGT
Cfr10I 807 ACCGGT [GA]^CCGG[CT]
1958 GCCGGT
DsaI 1646 CCATGG C^C[GA][CT]GG
2258 CCGTGG
Eco47III 1012 AGCGCT AGC^GCT
1135 AGCGCT
EcoT14I 1023 CCTTGG C^C[AT][AT]GG
1646 CCATGG
Hin1I 94 GACGCC G[GA]^CG[CT]C
231 GGCGCC
NspI 659 GCATGT [GA]CATG^[CT]
1760 GCATGC
PpuMI 1148 GGGACCT [GA]G^G[AT]CC[CT]
2416 GGGTCCT
SacI 529 GAGCTC GAGCT^C
1197 GAGCTC
SfcI 1809 CTACAG C^T[GA][CT]AG
2321 CTGCAG
SmlI 854 CTCAAG C^T[CT][GA]AG
1034 CTCGAG
StyI 1023 CCTTGG C^C[AT][AT]GG
1646 CCATGG
Tsp509I 555 AATT ^AATT
1714 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoO65I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII