Restriction map for
pFN21AE4237
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AcyI AgeI Aor51HI
ApaI AscI Asp718I AspI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BglI BsaHI BsiWI
BsmBI Bsp1407I BspDI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstXI
ClaI CpoI DraIII Eco47III EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 3117 GTATAC GT^[AC][GT]AC
AclI 1463 AACGTT AA^CGTT
AflII 1309 CTTAAG C^TTAAG
AflIII 2226 ACATGT A^C[GA][CT]GT
AhdI 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3050 GTGCAC G^TGCAC
AlwNI 881 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 582 CCTCAGG CC^T[ACGT]AGG
ApaLI 3050 GTGCAC G^TGCAC
AseI 1554 ATTAAT AT^TAAT
AsnI 1554 ATTAAT AT^TAAT
AspEI 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 213 CCTAGG C^CTAGG
BclI 2900 TGATCA T^GATCA
BfrI 1309 CTTAAG C^TTAAG
BglII 1560 AGATCT A^GATCT
BlnI 213 CCTAGG C^CTAGG
BlpI 2073 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2073 GCTCAGC GC^T[ACGT]AGC
BsaAI 3061 CACGTA [CT]AC^GT[GA]
BsaBI 1270 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 160 GAGACC GGTCTC(1/5)
BsgI 2809 GTGCAG GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsmI 2955 GCATTC GAATGC(1/-1)
BspHI 1590 TCATGA T^CATGA
BspLU11I 2226 ACATGT A^CATGT
BspMI 425 ACCTGC ACCTGC(4/8)
BsrBI 521 CCGCTC CCGCTC(-3/-3)
BsrDI 2990 CATTGC GCAATG(2/0)
Bst1107I 3117 GTATAC GTA^TAC
BstBI 3026 TTCGAA TT^CGAA
BstZ17I 3117 GTATAC GTA^TAC
Bsu36I 582 CCTCAGG CC^T[ACGT]AGG
CelII 2073 GCTCAGC GC^T[ACGT]AGC
DrdI 2708 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 8 CGGCCG C^GGCCG
Eam1105I 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
Eco81I 582 CCTCAGG CC^T[ACGT]AGG
EcoRV 2005 GATATC GAT^ATC
FbaI 2900 TGATCA T^GATCA
KspI 116 CCGCGG CCGC^GG
MamI 1270 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 16 GCCGGC GCC^GGC
NcoI 1056 CCATGG C^CATGG
NgoMIV 16 GCCGGC G^CCGGC
NspV 3026 TTCGAA TT^CGAA
PshBI 1554 ATTAAT AT^TAAT
Psp1406I 1463 AACGTT AA^CGTT
RcaI 1590 TCATGA T^CATGA
SacII 116 CCGCGG CCGC^GG
ScaI 1344 AGTACT AGT^ACT
SfuI 3026 TTCGAA TT^CGAA
SmlI 1309 CTTAAG C^T[CT][GA]AG
SphI 2049 GCATGC GCATG^C
StuI 984 AGGCCT AGG^CCT
Tsp45I 467 GTGAC ^GT[GC]AC
XbaI 2959 TCTAGA T^CTAGA
XcmI 529 CCAACTAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 66 CGCG CG^CG
117 CGCG
AccIII 1169 TCCGGA T^CCGGA
2940 TCCGGA
AspHI 2126 GAGCAC G[AT]GC[AT]^C
3050 GTGCAC
AvaI 382 CTCGGG C^[CT]CG[GA]G
448 CTCGGG
BalI 506 TGGCCA TGG^CCA
2255 TGGCCA
BanII 75 GGGCTC G[GA]GC[CT]^C
2848 GAGCCC
BbsI 728 GAAGAC GAAGAC(2/6)
997 GAAGAC
BpuAI 728 GAAGAC GAAGAC(2/6)
997 GAAGAC
BseAI 1169 TCCGGA T^CCGGA
2940 TCCGGA
BsiHKAI 2126 GAGCAC G[AT]GC[AT]^C
3050 GTGCAC
BsoBI 382 CTCGGG C^[CT]CG[GA]G
448 CTCGGG
BspEI 1169 TCCGGA T^CCGGA
2940 TCCGGA
BstUI 66 CGCG CG^CG
117 CGCG
CfoI 65 GCGC GCG^C
2019 GCGC
EcoNI 582 CCTCAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1767 CCTTCGTCAGG
EcoT14I 213 CCTAGG C^C[AT][AT]GG
1056 CCATGG
HhaI 65 GCGC GCG^C
2019 GCGC
HinP1I 65 GCGC G^CGC
2019 GCGC
HincII 516 GTTGAC GT[CT]^[GA]AC
679 GTCAAC
HindII 516 GTTGAC GT[CT]^[GA]AC
679 GTCAAC
MluNI 506 TGGCCA TGG^CCA
2255 TGGCCA
MroI 1169 TCCGGA T^CCGGA
2940 TCCGGA
MscI 506 TGGCCA TGG^CCA
2255 TGGCCA
MspA1I 71 CAGCGG C[AC]G^C[GT]G
116 CCGCGG
MvnI 66 CGCG CG^CG
117 CGCG
NspI 2049 GCATGC [GA]CATG^[CT]
2226 ACATGT
PpuMI 640 GGGTCCT [GA]G^G[AT]CC[CT]
2110 GGGTCCC
PstI 1113 CTGCAG CTGCA^G
1714 CTGCAG
SspI 1183 AATATT AAT^ATT
2574 AATATT
StyI 213 CCTAGG C^C[AT][AT]GG
1056 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI