##################################
# NO CUT ENZYMES (  230 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        ItaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        TseI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AhdI          146  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          174  AGCT                 AG^CT
AlwI          231  GATCC                GGATC(4/5)
AlwNI         173  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         146  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsmFI         144  GGGAC                GGGAC(10/14)
BsrDI          76  GCAATG               GCAATG(2/0)
BstXI          65  CCACTGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI           68  CTGAG                C^T[ACGT]AG
DpnII         231  GATC                 ^GATC
Eam1105I      146  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           93  CTCTTC               CTCTTC(1/4)
Ksp632I        93  CTCTTC               CTCTTC(1/4)
MboI          231  GATC                 ^GATC
MslI           66  CACTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         231  GATC                 ^GATC
Sau3AI        231  GATC                 ^GATC
SfcI          179  CTGTAG               C^T[GA][CT]AG
Tsp45I        103  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI           62  GTAC                 GT^AC
              169  GTAC                
MaeIII        103  GTCAC                ^GT[ACGT]AC
              113  GTTAC               
MboII          94  TCTTC                GAAGA(8/7)
               97  TCTTC               
MseI           58  TTAA                 T^TAA
              238  TTAA                
RsaI           62  GTAC                 GT^AC
              169  GTAC                
Tru9I          58  TTAA                 T^TAA
              238  TTAA                
Tsp509I        39  AATT                 ^AATT
              240  AATT                
TspRI          66  CACTG                CA[GC]TG[ACGT][ACGT]^
              165  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI