Restriction map for
pFN21AE6666
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# NO CUT ENZYMES ( 142 enzymes) #
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AccI AccII AccIII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV FauI FseI FspI
HaeII HaeIII HgaI HhaI HinP1I
HindIII KasI KspI MamI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 630 GACGTC GACGT^C
Acc65I 476 GGTACC G^GTACC
AcyI 630 GACGTC G[GA]^CG[CT]C
AluI 379 AGCT AG^CT
AocI 430 CCTCAGG CC^T[ACGT]AGG
AseI 408 ATTAAT AT^TAAT
AsnI 408 ATTAAT AT^TAAT
Asp718I 476 GGTACC G^GTACC
AspHI 85 GAGCAC G[AT]GC[AT]^C
AvaII 483 GGACC G^G[AT]CC
BamHI 856 GGATCC G^GATCC
BanII 230 GGGCTC G[GA]GC[CT]^C
BbvI 396 GCAGC GCAGC(8/12)
BclI 168 TGATCA T^GATCA
BglII 750 AGATCT A^GATCT
BsaHI 630 GACGTC G[GA]^CG[CT]C
BseRI 381 CTCCTC GAGGAG(10/8)
BsiHKAI 85 GAGCAC G[AT]GC[AT]^C
BsmBI 632 CGTCTC CGTCTC(1/5)
BssSI 835 CTCGTG CACGAG(-5/-1)
BstAPI 443 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 333 GGTGACC G^GT[ACGT]ACC
BstF5I 711 CATCC GGATG(2/0)
BstPI 333 GGTGACC G^GT[ACGT]ACC
BstXI 140 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 430 CCTCAGG CC^T[ACGT]AGG
Cac8I 135 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 483 GGACC G^G[ACGT]CC
DraI 763 TTTAAA TTT^AAA
DsaI 140 CCATGG C^C[GA][CT]GG
Eco81I 430 CCTCAGG CC^T[ACGT]AGG
EcoO65I 333 GGTGACC G^GT[ACGT]ACC
EcoT22I 124 ATGCAT ATGCA^T
FbaI 168 TGATCA T^GATCA
Fnu4HI 396 GCAGC GC^[ACGT]GC
FokI 711 CATCC GGATG(9/13)
Hin1I 630 GACGTC G[GA]^CG[CT]C
HincII 292 GTTAAC GT[CT]^[GA]AC
HindII 292 GTTAAC GT[CT]^[GA]AC
HpaI 292 GTTAAC GTT^AAC
ItaI 396 GCAGC GC^[ACGT]GC
KpnI 476 GGTACC GGTAC^C
MaeII 631 ACGT A^CGT
MaeIII 334 GTGAC ^GT[ACGT]AC
MfeI 593 CAATTG C^AATTG
MslI 306 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 593 CAATTG C^AATTG
NcoI 140 CCATGG C^CATGG
NsiI 124 ATGCAT ATGCA^T
NspI 122 ACATGC [GA]CATG^[CT]
PleI 607 GAGTC GAGTC(4/5)
PshBI 408 ATTAAT AT^TAAT
Sau96I 483 GGACC G^G[ACGT]CC
SbfI 327 CCTGCAGG CCTGCA^GG
SexAI 846 ACCTGGT A^CC[AT]GGT
SfaNI 364 GATGC GCATC(5/9)
SmlI 82 CTTGAG C^T[CT][GA]AG
Sse8387I 327 CCTGCAGG CCTGCA^GG
TfiI 470 GAATC G^A[AT]TC
TseI 396 GCAGC G^C[AT]GC
Tsp45I 334 GTGAC ^GT[GC]AC
XbaI 402 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 520 GAATTT [GA]^AATT[CT]
727 AAATTC
AfaI 225 GTAC GT^AC
477 GTAC
ApoI 520 GAATTT [GA]^AATT[CT]
727 AAATTC
BanI 435 GGTGCC G^G[CT][GA]CC
476 GGTACC
BcnI 96 CCGGG CC^[GC]GG
189 CCGGG
BfaI 44 CTAG C^TAG
403 CTAG
BpmI 205 CTCCAG CTGGAG(16/14)
287 CTCCAG
BsmAI 203 GTCTC GTCTC(1/5)
633 GTCTC
BspMI 623 ACCTGC ACCTGC(4/8)
330 GCAGGT
EarI 460 GAAGAG CTCTTC(1/4)
781 GAAGAG
EcoT14I 140 CCATGG C^C[AT][AT]GG
821 CCTTGG
HapII 96 CCGG C^CGG
189 CCGG
HinfI 470 GAATC G^A[ACGT]TC
607 GAGTC
HpaII 96 CCGG C^CGG
189 CCGG
Ksp632I 460 GAAGAG CTCTTC(1/4)
781 GAAGAG
MaeI 44 CTAG C^TAG
403 CTAG
MspI 96 CCGG C^CGG
189 CCGG
NciI 96 CCGGG CC^[GC]GG
189 CCGGG
PstI 328 CTGCAG CTGCA^G
625 CTGCAG
RsaI 225 GTAC GT^AC
477 GTAC
StyI 140 CCATGG C^C[AT][AT]GG
821 CCTTGG
TaqI 362 TCGA T^CGA
758 TCGA
TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^
774 CACTG
TthHB8I 362 TCGA T^CGA
758 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AlwI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstNI BstYI
DdeI DpnII EcoRII HphI MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI SfcI Tru9I Tsp509I XhoII