Restriction map for
pFN21AE6555
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MaeII MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 134 CGCG CG^CG
AcyI 67 GGCGTC G[GA]^CG[CT]C
AfaI 341 GTAC GT^AC
AgeI 154 ACCGGT A^CCGGT
Alw44I 127 GTGCAC G^TGCAC
AlwNI 238 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 228 CCTGAGG CC^T[ACGT]AGG
ApaLI 127 GTGCAC G^TGCAC
AspHI 127 GTGCAC G[AT]GC[AT]^C
BanII 97 GAGCCC G[GA]GC[CT]^C
BbvCI 303 CCTCAGC CCTCAGC(-5/-2)
BglI 4 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 78 GCTCAGC GC^T[ACGT]AGC
BpmI 181 CTGGAG CTGGAG(16/14)
Bpu1102I 78 GCTCAGC GC^T[ACGT]AGC
BsaBI 452 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 67 GGCGTC G[GA]^CG[CT]C
BsaWI 154 ACCGGT [AT]^CCGG[AT]
BsiHKAI 127 GTGCAC G[AT]GC[AT]^C
BspHI 255 TCATGA T^CATGA
BsrBI 76 CCGCTC CCGCTC(-3/-3)
BstUI 134 CGCG CG^CG
Bsu36I 228 CCTGAGG CC^T[ACGT]AGG
CelII 78 GCTCAGC GC^T[ACGT]AGC
CfoI 20 GCGC GCG^C
DraII 427 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 228 CCTGAGG CC^T[ACGT]AGG
EcoNI 224 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 427 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 19 GGCGCT [GA]GCGC^[CT]
HgaI 68 GCGTC GACGC(5/10)
HhaI 20 GCGC GCG^C
Hin1I 67 GGCGTC G[GA]^CG[CT]C
HinP1I 20 GCGC G^CGC
MaeIII 158 GTCAC ^GT[ACGT]AC
MamI 452 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 446 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 134 CGCG CG^CG
NaeI 64 GCCGGC GCC^GGC
NgoMIV 64 GCCGGC G^CCGGC
NruI 133 TCGCGA TCG^CGA
PinAI 154 ACCGGT A^CCGGT
PpuMI 427 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 255 TCATGA T^CATGA
RsaI 341 GTAC GT^AC
SapI 45 GCTCTTC GCTCTTC(1/4)
TfiI 269 GAATC G^A[AT]TC
Tsp45I 158 GTCAC ^GT[GC]AC
XcmI 327 CCAGGAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 120 GATCC GGATC(4/5)
87 GGATC
AvaII 234 GGACC G^G[AT]CC
428 GGACC
BcnI 5 CCCGG CC^[GC]GG
93 CCCGG
BsaJI 408 CCCAGG C^C[ACGT][ACGT]GG
409 CCAGGG
BsrFI 64 GCCGGC [GA]^CCGG[CT]
154 ACCGGT
BstNI 327 CCAGG CC^[AT]GG
409 CCAGG
BstYI 87 GGATCT [GA]^GATC[CT]
119 AGATCC
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
154 ACCGGT
EarI 46 CTCTTC CTCTTC(1/4)
174 GAAGAG
EcoRII 327 CCAGG ^CC[AT]GG
409 CCAGG
Ksp632I 46 CTCTTC CTCTTC(1/4)
174 GAAGAG
MboII 174 GAAGA GAAGA(8/7)
47 TCTTC
MflI 87 GGATCT [GA]^GATC[CT]
119 AGATCC
MspA1I 81 CAGCTG C[AC]G^C[GT]G
247 CAGCTG
MvaI 327 CCAGG CC^[AT]GG
409 CCAGG
NciI 5 CCCGG CC^[GC]GG
93 CCCGG
NlaIII 165 CATG CATG^
256 CATG
PleI 177 GAGTC GAGTC(4/5)
252 GAGTC
PstI 244 CTGCAG CTGCA^G
350 CTGCAG
PvuII 81 CAGCTG CAG^CTG
247 CAGCTG
SfcI 244 CTGCAG C^T[GA][CT]AG
350 CTGCAG
XhoII 87 GGATCT [GA]^GATC[CT]
119 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BmyI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
Cac8I Cfr13I DdeI DpnII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MboI MnlI MspI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI