Restriction map for
pFN21AE6390
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BbrPI BbsI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI KspI MamI MluI
MroI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 784 CGCG CG^CG
AciI 995 CCGC CCGC(-3/-1)
AcyI 1054 GGCGCC G[GA]^CG[CT]C
Aor51HI 1243 AGCGCT AGC^GCT
AspI 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 659 TGGCCA TGG^CCA
BamHI 1068 GGATCC G^GATCC
BanI 1054 GGCGCC G^G[CT][GA]CC
BanII 341 GAGCCC G[GA]GC[CT]^C
BbeI 1054 GGCGCC GGCGC^C
BbvCI 1154 CCTCAGC CCTCAGC(-5/-2)
BmyI 341 GAGCCC G[AGT]GC[ACT]^C
BsaHI 1054 GGCGCC G[GA]^CG[CT]C
BsaI 565 GAGACC GGTCTC(1/5)
BsaWI 63 ACCGGA [AT]^CCGG[AT]
BseRI 1037 CTCCTC GAGGAG(10/8)
BsmI 1117 GCATTC GAATGC(1/-1)
Bsp1286I 341 GAGCCC G[AGT]GC[ACT]^C
BsrDI 735 CATTGC GCAATG(2/0)
BstUI 784 CGCG CG^CG
Cac8I 378 GCAAGC GC[ACGT]^[ACGT]GC
DsaI 1049 CCATGG C^C[GA][CT]GG
EaeI 659 TGGCCA [CT]^GGCC[GA]
Eco47III 1243 AGCGCT AGC^GCT
FauI 994 CCCGC CCCGC(4/6)
HapII 64 CCGG C^CGG
Hin1I 1054 GGCGCC G[GA]^CG[CT]C
HpaII 64 CCGG C^CGG
KasI 1054 GGCGCC G^GCGCC
MaeII 999 ACGT A^CGT
MfeI 248 CAATTG C^AATTG
MluNI 659 TGGCCA TGG^CCA
MscI 659 TGGCCA TGG^CCA
MslI 1095 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1177 CAGCTG C[AC]G^C[GT]G
MspI 64 CCGG C^CGG
MunI 248 CAATTG C^AATTG
MvnI 784 CGCG CG^CG
MwoI 634 GCAGTCATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 1054 GGCGCC GG^CGCC
NcoI 1049 CCATGG C^CATGG
PflFI 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1045 GAGTC GAGTC(4/5)
PshAI 567 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1177 CAGCTG CAG^CTG
SapI 114 GCTCTTC GCTCTTC(1/4)
SfoI 1054 GGCGCC GGC^GCC
TaqI 930 TCGA T^CGA
Tth111I 673 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 930 TCGA T^CGA
XcmI 345 CCAGAAACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AfaI 617 GTAC GT^AC
825 GTAC
AvaII 414 GGACC G^G[AT]CC
991 GGACC
BclI 362 TGATCA T^GATCA
449 TGATCA
BfaI 961 CTAG C^TAG
1247 CTAG
BpmI 877 CTCCAG CTGGAG(16/14)
284 CTGGAG
BsgI 38 GTGCAG GTGCAG(16/14)
632 GTGCAG
BsmAI 565 GAGAC GTCTC(1/5)
443 GTCTC
BsmFI 413 GGGAC GGGAC(10/14)
590 GTCCC
BssKI 665 CCAGG ^CC[ACGT]GG
888 CCTGG
BstNI 665 CCAGG CC^[AT]GG
888 CCTGG
CfoI 1055 GCGC GCG^C
1244 GCGC
DraII 413 GGGACCC [GA]G^G[ACGT]CC[CT]
990 AGGACCC
EarI 115 CTCTTC CTCTTC(1/4)
847 CTCTTC
EcoO109I 413 GGGACCC [GA]G^G[ACGT]CC[CT]
990 AGGACCC
EcoRII 665 CCAGG ^CC[AT]GG
888 CCTGG
EcoT14I 774 CCAAGG C^C[AT][AT]GG
1049 CCATGG
FbaI 362 TGATCA T^GATCA
449 TGATCA
HaeII 1054 GGCGCC [GA]GCGC^[CT]
1243 AGCGCT
HhaI 1055 GCGC GCG^C
1244 GCGC
HinP1I 1055 GCGC G^CGC
1244 GCGC
Ksp632I 115 CTCTTC CTCTTC(1/4)
847 CTCTTC
MaeI 961 CTAG C^TAG
1247 CTAG
MvaI 665 CCAGG CC^[AT]GG
888 CCTGG
PpuMI 413 GGGACCC [GA]G^G[AT]CC[CT]
990 AGGACCC
PstI 173 CTGCAG CTGCA^G
419 CTGCAG
RsaI 617 GTAC GT^AC
825 GTAC
ScrFI 665 CCAGG CC^[ACGT]GG
888 CCTGG
SfaNI 918 GATGC GCATC(5/9)
5 GCATC
StuI 581 AGGCCT AGG^CCT
1017 AGGCCT
StyI 774 CCAAGG C^C[AT][AT]GG
1049 CCATGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BbvI BsaJI BsiYI BslI BspHI
BsrI BstF5I BstYI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII NlaIV
PflMI RcaI Sau3AI Sau96I SfcI
TfiI Tru9I TseI Tsp509I TspRI
Van91I XhoII