Restriction map for
pFN21AE2459
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrI BlnI
BpuAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccII 82 CGCG CG^CG
AcyI 29 GGCGTC G[GA]^CG[CT]C
AflIII 130 ACGTGT A^C[GA][CT]GT
AlwNI 1350 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1444 GAGCAC G[AT]GC[AT]^C
AvaII 228 GGACC G^G[AT]CC
BalI 1491 TGGCCA TGG^CCA
BbrPI 129 CACGTG CAC^GTG
BcnI 404 CCCGG CC^[GC]GG
BglI 971 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1234 AGATCT A^GATCT
BlpI 529 GCTGAGC GC^T[ACGT]AGC
BmyI 1444 GAGCAC G[AGT]GC[ACT]^C
BpmI 107 CTGGAG CTGGAG(16/14)
Bpu1102I 529 GCTGAGC GC^T[ACGT]AGC
BsaAI 129 CACGTG [CT]AC^GT[GA]
BsaHI 29 GGCGTC G[GA]^CG[CT]C
BsaJI 845 CCTTGG C^C[ACGT][ACGT]GG
BseRI 394 GAGGAG GAGGAG(10/8)
BsiHKAI 1444 GAGCAC G[AT]GC[AT]^C
BsmAI 550 GTCTC GTCTC(1/5)
BsmI 1458 GAATGC GAATGC(1/-1)
Bsp1286I 1444 GAGCAC G[AGT]GC[ACT]^C
BspDI 1001 ATCGAT AT^CGAT
BstUI 82 CGCG CG^CG
BstXI 1200 CCAAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1234 AGATCT [GA]^GATC[CT]
CelII 529 GCTGAGC GC^T[ACGT]AGC
CfoI 83 GCGC GCG^C
ClaI 1001 ATCGAT AT^CGAT
DraIII 1447 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 583 GAAGAG CTCTTC(1/4)
EcoNI 387 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 845 CCTTGG C^C[AT][AT]GG
EcoT22I 700 ATGCAT ATGCA^T
HapII 405 CCGG C^CGG
HgaI 30 GCGTC GACGC(5/10)
HhaI 83 GCGC GCG^C
Hin1I 29 GGCGTC G[GA]^CG[CT]C
HinP1I 83 GCGC G^CGC
HincII 1361 GTCAAC GT[CT]^[GA]AC
HindII 1361 GTCAAC GT[CT]^[GA]AC
HpaII 405 CCGG C^CGG
Ksp632I 583 GAAGAG CTCTTC(1/4)
MaeIII 515 GTGAC ^GT[ACGT]AC
MfeI 481 CAATTG C^AATTG
MflI 1234 AGATCT [GA]^GATC[CT]
MluNI 1491 TGGCCA TGG^CCA
MscI 1491 TGGCCA TGG^CCA
MslI 1183 CACAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 104 CAGCTG C[AC]G^C[GT]G
MspI 405 CCGG C^CGG
MunI 481 CAATTG C^AATTG
MvnI 82 CGCG CG^CG
NciI 404 CCCGG CC^[GC]GG
NsiI 700 ATGCAT ATGCA^T
PmaCI 129 CACGTG CAC^GTG
PmlI 129 CACGTG CAC^GTG
PshAI 1354 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 101 CTGCAG CTGCA^G
PvuII 104 CAGCTG CAG^CTG
SphI 922 GCATGC GCATG^C
StyI 845 CCTTGG C^C[AT][AT]GG
Tsp45I 515 GTGAC ^GT[GC]AC
XbaI 1126 TCTAGA T^CTAGA
XhoII 1234 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 909 GGATC GGATC(4/5)
1024 GGATC
BclI 207 TGATCA T^GATCA
867 TGATCA
BsaBI 862 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1088 GATCAACATC
BsiYI 100 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
387 CCTTCGTGAGG
BslI 100 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
387 CCTTCGTGAGG
BspHI 1440 TCATGA T^CATGA
1473 TCATGA
DraII 978 AGGCCCC [GA]G^G[ACGT]CC[CT]
1390 AGGGCCT
EaeI 802 TGGCCG [CT]^GGCC[GA]
1491 TGGCCA
EcoO109I 978 AGGCCCC [GA]G^G[ACGT]CC[CT]
1390 AGGGCCT
FbaI 207 TGATCA T^GATCA
867 TGATCA
HphI 514 GGTGA GGTGA(8/7)
334 TCACC
MaeII 130 ACGT A^CGT
1365 ACGT
MamI 862 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1088 GATCAACATC
NlaIV 979 GGCCCC GG[ACGT]^[ACGT]CC
1210 GGCTCC
RcaI 1440 TCATGA T^CATGA
1473 TCATGA
SfcI 101 CTGCAG C^T[GA][CT]AG
732 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I