Restriction map for
pFN21AE6277
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MroI
MspA1I MunI MvaI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 536 AACGTT AA^CGTT
AcsI 525 AAATTC [GA]^AATT[CT]
ApoI 525 AAATTC [GA]^AATT[CT]
AspHI 665 GTGCTC G[AT]GC[AT]^C
AvaII 655 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 32 GGTGCC G^G[CT][GA]CC
BcnI 67 CCCGG CC^[GC]GG
BglII 647 AGATCT A^GATCT
BmyI 665 GTGCTC G[AGT]GC[ACT]^C
BpmI 254 CTGGAG CTGGAG(16/14)
BsaJI 202 CCATGG C^C[ACGT][ACGT]GG
BseRI 86 GAGGAG GAGGAG(10/8)
BsgI 80 GTGCAG GTGCAG(16/14)
BsiHKAI 665 GTGCTC G[AT]GC[AT]^C
BsmAI 639 GTCTC GTCTC(1/5)
BsmFI 656 GTCCC GGGAC(10/14)
Bsp1286I 665 GTGCTC G[AGT]GC[ACT]^C
BsrFI 29 GCCGGT [GA]^CCGG[CT]
BssKI 67 CCCGG ^CC[ACGT]GG
BstYI 647 AGATCT [GA]^GATC[CT]
Cfr10I 29 GCCGGT [GA]^CCGG[CT]
Cfr13I 655 GGTCC G^G[ACGT]CC
DsaI 202 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoT14I 202 CCATGG C^C[AT][AT]GG
MflI 647 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 64 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 67 CCCGG CC^[GC]GG
NcoI 202 CCATGG C^CATGG
NlaIII 203 CATG CATG^
PflMI 197 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 536 AACGTT AA^CGTT
Sau96I 655 GGTCC G^G[ACGT]CC
ScrFI 67 CCCGG CC^[ACGT]GG
SspI 436 AATATT AAT^ATT
StyI 202 CCATGG C^C[AT][AT]GG
Van91I 197 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 647 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 36 CCGC CCGC(-3/-1)
103 CCGC
AluI 258 AGCT AG^CT
603 AGCT
BfaI 74 CTAG C^TAG
149 CTAG
BsrI 182 CCAGT ACTGG(1/-1)
418 CCAGT
DdeI 293 CTGAG C^T[ACGT]AG
313 CTTAG
HaeIII 3 GGCC GG^CC
28 GGCC
HapII 30 CCGG C^CGG
68 CCGG
HinfI 250 GATTC G^A[ACGT]TC
382 GAATC
HpaII 30 CCGG C^CGG
68 CCGG
HphI 415 TCACC GGTGA(8/7)
460 TCACC
MaeI 74 CTAG C^TAG
149 CTAG
MaeII 132 ACGT A^CGT
537 ACGT
MboII 621 GAAGA GAAGA(8/7)
17 TCTTC
MseI 496 TTAA T^TAA
541 TTAA
MspI 30 CCGG C^CGG
68 CCGG
NlaIV 32 GGTGCC GG[ACGT]^[ACGT]CC
655 GGTCCC
TfiI 250 GATTC G^A[AT]TC
382 GAATC
Tru9I 496 TTAA T^TAA
541 TTAA
TspRI 394 CAGTG CA[GC]TG[ACGT][ACGT]^
419 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BbvI BsiYI BslI BstF5I DpnII
Fnu4HI FokI ItaI MboI MnlI
MwoI NdeII Sau3AI TseI Tsp509I