Restriction map for
pFN21AE5170
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI BstYI Bsu36I
CelII ClaI CpoI DdeI DpnII
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
HphI KasI KpnI KspI MamI
MboI MfeI MflI MluNI MroI
MscI MunI NarI NcoI NdeI
NdeII NheI NlaIII NlaIV NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 336 GTATAC GT^[AC][GT]AC
AcsI 381 GAATTC [GA]^AATT[CT]
AcyI 59 GACGCC G[GA]^CG[CT]C
AflII 466 CTTAAG C^TTAAG
AflIII 213 ACGCGT A^C[GA][CT]GT
ApoI 381 GAATTC [GA]^AATT[CT]
AvaI 250 CTCGAG C^[CT]CG[GA]G
AvaII 294 GGACC G^G[AT]CC
BcgI 147 CGAAGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 73 CCCGG CC^[GC]GG
BfaI 106 CTAG C^TAG
BfrI 466 CTTAAG C^TTAAG
BmyI 328 GTGCCC G[AGT]GC[ACT]^C
BsaHI 59 GACGCC G[GA]^CG[CT]C
BsiEI 9 CGGCCG CG[GA][CT]^CG
BsmFI 435 GGGAC GGGAC(10/14)
BsoBI 250 CTCGAG C^[CT]CG[GA]G
Bsp1286I 328 GTGCCC G[AGT]GC[ACT]^C
BsrFI 274 GCCGGC [GA]^CCGG[CT]
Bst1107I 336 GTATAC GTA^TAC
BstZ17I 336 GTATAC GTA^TAC
Cfr10I 274 GCCGGC [GA]^CCGG[CT]
DsaI 297 CCACGG C^C[GA][CT]GG
EaeI 9 CGGCCG [CT]^GGCC[GA]
EagI 9 CGGCCG C^GGCCG
EarI 4 CTCTTC CTCTTC(1/4)
EclXI 9 CGGCCG C^GGCCG
Eco52I 9 CGGCCG C^GGCCG
EcoRI 381 GAATTC G^AATTC
EcoT14I 290 CCAAGG C^C[AT][AT]GG
Hin1I 59 GACGCC G[GA]^CG[CT]C
Ksp632I 4 CTCTTC CTCTTC(1/4)
MaeI 106 CTAG C^TAG
MaeII 197 ACGT A^CGT
MaeIII 411 GTTAC ^GT[ACGT]AC
MboII 5 TCTTC GAAGA(8/7)
MluI 213 ACGCGT A^CGCGT
MseI 467 TTAA T^TAA
MspA1I 19 CCGCTG C[AC]G^C[GT]G
NaeI 274 GCCGGC GCC^GGC
NciI 73 CCCGG CC^[GC]GG
NgoMIV 274 GCCGGC G^CCGGC
PaeR7I 250 CTCGAG C^TCGAG
PstI 442 CTGCAG CTGCA^G
SapI 3 GCTCTTC GCTCTTC(1/4)
SfcI 442 CTGCAG C^T[GA][CT]AG
StyI 290 CCAAGG C^C[AT][AT]GG
Tru9I 467 TTAA T^TAA
Tsp509I 382 AATT ^AATT
TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 250 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BseRI 136 GAGGAG GAGGAG(10/8)
253 GAGGAG
BstF5I 15 CATCC GGATG(2/0)
348 GGATG
FokI 15 CATCC GGATG(9/13)
348 GGATG
HapII 74 CCGG C^CGG
275 CCGG
HinfI 80 GATTC G^A[ACGT]TC
471 GATTC
HpaII 74 CCGG C^CGG
275 CCGG
MslI 33 CATACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
226 CACTTCTATG
MspI 74 CCGG C^CGG
275 CCGG
PflMI 297 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCAGGACTTGG
SmlI 250 CTCGAG C^T[CT][GA]AG
466 CTTAAG
StuI 360 AGGCCT AGG^CCT
462 AGGCCT
TaqI 53 TCGA T^CGA
251 TCGA
TfiI 80 GATTC G^A[AT]TC
471 GATTC
TthHB8I 53 TCGA T^CGA
251 TCGA
Van91I 297 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCAGGACTTGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AccII AciI AluI BbvI BpmI
BsaJI BsiYI BslI BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeIII HgaI HhaI
HinP1I ItaI MnlI MvaI MvnI
MwoI Sau96I ScrFI SfaNI TseI