Restriction map for
pFN21AE6391
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbvCI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI MaeI MamI
MflI MluI MluNI MroI MscI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 340 GTCTAC GT^[AC][GT]AC
AccII 109 CGCG CG^CG
Alw44I 277 GTGCAC G^TGCAC
AlwNI 409 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 277 GTGCAC G^TGCAC
AvaI 1208 CTCGGG C^[CT]CG[GA]G
BanII 356 GAGCCC G[GA]GC[CT]^C
BbsI 1141 GTCTTC GAAGAC(2/6)
BcgI 1172 GCACACCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 95 TGATCA T^GATCA
BcnI 150 CCGGG CC^[GC]GG
BpuAI 1141 GTCTTC GAAGAC(2/6)
BseRI 76 GAGGAG GAGGAG(10/8)
BsmAI 961 GAGAC GTCTC(1/5)
BsoBI 1208 CTCGGG C^[CT]CG[GA]G
Bsp1407I 862 TGTACA T^GTACA
BsrGI 862 TGTACA T^GTACA
BsrI 753 CCAGT ACTGG(1/-1)
BstAPI 1110 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 109 CGCG CG^CG
DsaI 108 CCGCGG C^C[GA][CT]GG
EcoNI 423 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 110 GCGGG CCCGC(4/6)
FbaI 95 TGATCA T^GATCA
HaeII 410 AGCGCC [GA]GCGC^[CT]
HgaI 1139 GCGTC GACGC(5/10)
HindIII 18 AAGCTT A^AGCTT
KspI 108 CCGCGG CCGC^GG
MfeI 221 CAATTG C^AATTG
MslI 828 CATAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 221 CAATTG C^AATTG
MvnI 109 CGCG CG^CG
NciI 150 CCGGG CC^[GC]GG
PleI 739 GACTC GAGTC(4/5)
PpuMI 918 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 715 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 156 CAGCTG CAG^CTG
SacII 108 CCGCGG CCGC^GG
SbfI 360 CCTGCAGG CCTGCA^GG
SfaNI 1110 GCATC GCATC(5/9)
SphI 728 GCATGC GCATG^C
Sse8387I 360 CCTGCAGG CCTGCA^GG
SspBI 862 TGTACA T^GTACA
Tsp45I 1214 GTGAC ^GT[GC]AC
XcmI 1187 CCAGGTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 863 GTAC GT^AC
910 GTAC
AflIII 281 ACACGT A^C[GA][CT]GT
1153 ACGTGT
AlwI 573 GGATC GGATC(4/5)
1121 GGATC
AvaII 147 GGTCC G^G[AT]CC
919 GGACC
BbrPI 791 CACGTG CAC^GTG
1152 CACGTG
BsaAI 791 CACGTG [CT]AC^GT[GA]
1152 CACGTG
BsmFI 152 GGGAC GGGAC(10/14)
918 GGGAC
BsmI 192 GAATGC GAATGC(1/-1)
833 GAATGC
BsrFI 137 GCCGGC [GA]^CCGG[CT]
404 GCCGGC
BssSI 73 CACGAG CACGAG(-5/-1)
1180 CTCGTG
CfoI 85 GCGC GCG^C
411 GCGC
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
404 GCCGGC
DraII 419 AGGCCCT [GA]G^G[ACGT]CC[CT]
918 GGGACCT
EcoO109I 419 AGGCCCT [GA]G^G[ACGT]CC[CT]
918 GGGACCT
HhaI 85 GCGC GCG^C
411 GCGC
HinP1I 85 GCGC G^CGC
411 GCGC
HphI 1213 GGTGA GGTGA(8/7)
801 TCACC
NaeI 137 GCCGGC GCC^GGC
404 GCCGGC
NgoMIV 137 GCCGGC G^CCGGC
404 GCCGGC
NspI 600 ACATGC [GA]CATG^[CT]
728 GCATGC
PmaCI 791 CACGTG CAC^GTG
1152 CACGTG
PmlI 791 CACGTG CAC^GTG
1152 CACGTG
RsaI 863 GTAC GT^AC
910 GTAC
TfiI 546 GATTC G^A[AT]TC
778 GATTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
BbvI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI Tru9I
TseI Tsp509I TspRI