Restriction map for
pFN21AE6667
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 190 AAATTC [GA]^AATT[CT]
AfaI 135 GTAC GT^AC
ApoI 190 AAATTC [GA]^AATT[CT]
Asp700I 483 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 866 GGCACC G^G[CT][GA]CC
BbsI 424 GAAGAC GAAGAC(2/6)
BcgI 791 CGAAAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 642 GTATCC GTATCC(6/5)
BclI 326 TGATCA T^GATCA
BcnI 825 CCGGG CC^[GC]GG
BpuAI 424 GAAGAC GAAGAC(2/6)
BseRI 418 GAGGAG GAGGAG(10/8)
BsgI 161 CTGCAC GTGCAG(16/14)
BstF5I 852 CATCC GGATG(2/0)
CfoI 392 GCGC GCG^C
DpnII 327 GATC ^GATC
DraII 248 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 401 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 248 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 397 CCCGC CCCGC(4/6)
FbaI 326 TGATCA T^GATCA
FokI 852 CATCC GGATG(9/13)
HaeII 391 GGCGCT [GA]GCGC^[CT]
HapII 825 CCGG C^CGG
HgaI 582 GACGC GACGC(5/10)
HhaI 392 GCGC GCG^C
HinP1I 392 GCGC G^CGC
HpaII 825 CCGG C^CGG
MaeII 620 ACGT A^CGT
MboI 327 GATC ^GATC
MspI 825 CCGG C^CGG
NciI 825 CCGGG CC^[GC]GG
NdeII 327 GATC ^GATC
NspI 467 ACATGC [GA]CATG^[CT]
PleI 509 GAGTC GAGTC(4/5)
PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 135 GTAC GT^AC
Sau3AI 327 GATC ^GATC
SfaNI 798 GATGC GCATC(5/9)
SmlI 878 CTCAAG C^T[CT][GA]AG
StuI 819 AGGCCT AGG^CCT
TfiI 205 GATTC G^A[AT]TC
Tsp509I 191 AATT ^AATT
XmnI 483 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 29 GGACC G^G[AT]CC
249 GGACC
BanII 127 GAGCCC G[GA]GC[CT]^C
542 GAGCCC
BfaI 38 CTAG C^TAG
506 CTAG
BmyI 127 GAGCCC G[AGT]GC[ACT]^C
542 GAGCCC
BpmI 515 CTGGAG CTGGAG(16/14)
539 CTGGAG
BsmFI 464 GGGAC GGGAC(10/14)
580 GGGAC
Bsp1286I 127 GAGCCC G[AGT]GC[ACT]^C
542 GAGCCC
Cac8I 309 GCAGGC GC[ACGT]^[ACGT]GC
400 GCCTGC
EarI 90 CTCTTC CTCTTC(1/4)
95 CTCTTC
HinfI 205 GATTC G^A[ACGT]TC
509 GAGTC
Ksp632I 90 CTCTTC CTCTTC(1/4)
95 CTCTTC
MaeI 38 CTAG C^TAG
506 CTAG
MseI 521 TTAA T^TAA
605 TTAA
MslI 709 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
789 CACGAAAATG
Tru9I 521 TTAA T^TAA
605 TTAA
TspRI 273 CAGTG CA[GC]TG[ACGT][ACGT]^
366 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstNI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HphI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI