Restriction map for
pFN21AE6287
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmFI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII Hin1I HindIII HpaI
HphI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflIII 596 ACATGT A^C[GA][CT]GT
AluI 848 AGCT AG^CT
AlwI 856 GATCC GGATC(4/5)
AocI 135 CCTCAGG CC^T[ACGT]AGG
ApaI 302 GGGCCC GGGCC^C
Asp700I 246 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 52 GTGCTC G[AT]GC[AT]^C
AvaI 68 CTCGGG C^[CT]CG[GA]G
BalI 809 TGGCCA TGG^CCA
BbsI 580 GTCTTC GAAGAC(2/6)
BbvCI 639 CCTCAGC CCTCAGC(-5/-2)
BcgI 98 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 661 CTAG C^TAG
BpuAI 580 GTCTTC GAAGAC(2/6)
BseRI 267 CTCCTC GAGGAG(10/8)
BsgI 818 GTGCAG GTGCAG(16/14)
BsiEI 98 CGACCG CG[GA][CT]^CG
BsiHKAI 52 GTGCTC G[AT]GC[AT]^C
BsmBI 220 CGTCTC CGTCTC(1/5)
BsmI 82 GAATGC GAATGC(1/-1)
BsoBI 68 CTCGGG C^[CT]CG[GA]G
BspLU11I 596 ACATGT A^CATGT
BspMI 167 ACCTGC ACCTGC(4/8)
BsrBI 65 CCGCTC CCGCTC(-3/-3)
BsrDI 684 CATTGC GCAATG(2/0)
BstAPI 699 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 855 AGATCC [GA]^GATC[CT]
Bsu36I 135 CCTCAGG CC^T[ACGT]AGG
CpoI 61 CGGACCG CG^G[AT]CCG
EarI 278 CTCTTC CTCTTC(1/4)
Eco81I 135 CCTCAGG CC^T[ACGT]AGG
EcoT14I 738 CCTTGG C^C[AT][AT]GG
EcoT22I 566 ATGCAT ATGCA^T
FauI 27 GCGGG CCCGC(4/6)
HgaI 702 GACGC GACGC(5/10)
HincII 471 GTCAAC GT[CT]^[GA]AC
HindII 471 GTCAAC GT[CT]^[GA]AC
HinfI 587 GAATC G^A[ACGT]TC
Ksp632I 278 CTCTTC CTCTTC(1/4)
MaeI 661 CTAG C^TAG
MflI 855 AGATCC [GA]^GATC[CT]
MluNI 809 TGGCCA TGG^CCA
MscI 809 TGGCCA TGG^CCA
NruI 78 TCGCGA TCG^CGA
NsiI 566 ATGCAT ATGCA^T
NspI 596 ACATGT [GA]CATG^[CT]
RsrII 61 CGGACCG CG^G[AT]CCG
ScaI 237 AGTACT AGT^ACT
SmlI 872 CTCAAG C^T[CT][GA]AG
StyI 738 CCTTGG C^C[AT][AT]GG
TfiI 587 GAATC G^A[AT]TC
XhoII 855 AGATCC [GA]^GATC[CT]
XmnI 246 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 79 CGCG CG^CG
102 CGCG
AfaI 165 GTAC GT^AC
238 GTAC
AlwNI 346 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
700 CAGACGCTG
AvaII 62 GGACC G^G[AT]CC
72 GGTCC
BanII 148 GAGCCC G[GA]GC[CT]^C
302 GGGCCC
BcnI 10 CCCGG CC^[GC]GG
15 CCCGG
BsaJI 689 CCCTGG C^C[ACGT][ACGT]GG
738 CCTTGG
BsiYI 131 CCAGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
639 CCTCAGCATGG
BslI 131 CCAGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
639 CCTCAGCATGG
BsmAI 21 GTCTC GTCTC(1/5)
221 GTCTC
BstF5I 569 CATCC GGATG(2/0)
3 GGATG
BstUI 79 CGCG CG^CG
102 CGCG
Cac8I 27 GCGGGC GC[ACGT]^[ACGT]GC
130 GCCAGC
CfoI 103 GCGC GCG^C
113 GCGC
DpnII 768 GATC ^GATC
856 GATC
DraII 302 GGGCCCT [GA]G^G[ACGT]CC[CT]
347 AGGGCCT
EaeI 44 CGGCCA [CT]^GGCC[GA]
809 TGGCCA
EcoO109I 302 GGGCCCT [GA]G^G[ACGT]CC[CT]
347 AGGGCCT
FokI 569 CATCC GGATG(9/13)
3 GGATG
HhaI 103 GCGC GCG^C
113 GCGC
HinP1I 103 GCGC G^CGC
113 GCGC
MboI 768 GATC ^GATC
856 GATC
MvnI 79 CGCG CG^CG
102 CGCG
NciI 10 CCCGG CC^[GC]GG
15 CCCGG
NdeII 768 GATC ^GATC
856 GATC
PstI 521 CTGCAG CTGCA^G
697 CTGCAG
RsaI 165 GTAC GT^AC
238 GTAC
Sau3AI 768 GATC ^GATC
856 GATC
SfcI 521 CTGCAG C^T[GA][CT]AG
697 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI BbvI BmyI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MaeIII MboII MnlI MslI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI Tsp509I TspRI