##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          137  AAATTC               [GA]^AATT[CT]
AlwI          105  GGATC                GGATC(4/5)
ApoI          137  AAATTC               [GA]^AATT[CT]
Asp700I       213  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          13  GGACC                G^G[AT]CC
BbsI           41  GTCTTC               GAAGAC(2/6)
BfaI          166  CTAG                 C^TAG
BpmI           82  CTGGAG               CTGGAG(16/14)
BpuAI          41  GTCTTC               GAAGAC(2/6)
BsaJI         183  CCTTGG               C^C[ACGT][ACGT]GG
BsgI           87  GTGCAG               GTGCAG(16/14)
BsiYI          75  CCTACTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           75  CCTACTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         147  GTCTC                GTCTC(1/5)
BssKI          10  CCTGG                ^CC[ACGT]GG
BstAPI         72  GCACCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          10  CCTGG                CC^[AT]GG
Cac8I         240  GCTGGC               GC[ACGT]^[ACGT]GC
DpnII         106  GATC                 ^GATC
EcoRII         10  CCTGG                ^CC[AT]GG
EcoT14I       183  CCTTGG               C^C[AT][AT]GG
HaeIII        243  GGCC                 GG^CC
HinfI         253  GAGTC                G^A[ACGT]TC
MaeI          166  CTAG                 C^TAG
MaeIII         25  GTTAC                ^GT[ACGT]AC
MboI          106  GATC                 ^GATC
MboII          42  TCTTC                GAAGA(8/7)
MseI           68  TTAA                 T^TAA
MvaI           10  CCTGG                CC^[AT]GG
NdeII         106  GATC                 ^GATC
PleI          253  GAGTC                GAGTC(4/5)
PstI          111  CTGCAG               CTGCA^G
Sau3AI        106  GATC                 ^GATC
ScrFI          10  CCTGG                CC^[ACGT]GG
SfcI          111  CTGCAG               C^T[GA][CT]AG
StyI          183  CCTTGG               C^C[AT][AT]GG
Tru9I          68  TTAA                 T^TAA
Tsp509I       138  AATT                 ^AATT
XmnI          213  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI          164  AGCT                 AG^CT
              215  AGCT                
BbvI          113  GCAGC                GCAGC(8/12)
              162  GCAGC               
BsrI          127  CCAGT                ACTGG(1/-1)
              246  CCAGT               
Cfr13I         13  GGACC                G^G[ACGT]CC
              243  GGCCC               
DdeI          149  CTCAG                C^T[ACGT]AG
              158  CTGAG               
Fnu4HI        113  GCAGC                GC^[ACGT]GC
              162  GCAGC               
ItaI          113  GCAGC                GC^[ACGT]GC
              162  GCAGC               
NlaIV           6  GGAGCC               GG[ACGT]^[ACGT]CC
               13  GGACCC              
Sau96I         13  GGACC                G^G[ACGT]CC
              243  GGCCC               
TseI          113  GCAGC                G^C[AT]GC
              162  GCAGC               
TspRI          90  CAGTG                CA[GC]TG[ACGT][ACGT]^
              109  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MnlI        MwoI        NlaIII