##################################
# NO CUT ENZYMES (  221 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        ItaI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MscI        MslI        MspA1I      
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TseI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccIII         84  TCCGGA               T^CCGGA
AciI           60  CCGC                 CCGC(-3/-1)
AcsI          119  AAATTC               [GA]^AATT[CT]
ApoI          119  AAATTC               [GA]^AATT[CT]
BsaWI          84  TCCGGA               [AT]^CCGG[AT]
BseAI          84  TCCGGA               T^CCGGA
BspEI          84  TCCGGA               T^CCGGA
BssKI         105  CCAGG                ^CC[ACGT]GG
BstF5I        111  GGATG                GGATG(2/0)
BstNI         105  CCAGG                CC^[AT]GG
DraI           70  TTTAAA               TTT^AAA
EarI           20  CTCTTC               CTCTTC(1/4)
EcoRII        105  CCAGG                ^CC[AT]GG
FauI           59  CCCGC                CCCGC(4/6)
FokI          111  GGATG                GGATG(9/13)
HapII          85  CCGG                 C^CGG
HpaII          85  CCGG                 C^CGG
Ksp632I        20  CTCTTC               CTCTTC(1/4)
MboII          21  TCTTC                GAAGA(8/7)
MroI           84  TCCGGA               T^CCGGA
MseI           71  TTAA                 T^TAA
MspI           85  CCGG                 C^CGG
MvaI          105  CCAGG                CC^[AT]GG
NdeI          200  CATATG               CA^TATG
NlaIII        174  CATG                 CATG^
SapI           19  GCTCTTC              GCTCTTC(1/4)
ScrFI         105  CCAGG                CC^[ACGT]GG
SfaNI         151  GATGC                GCATC(5/9)
Tru9I          71  TTAA                 T^TAA
Tsp509I       120  AATT                 ^AATT
XcmI          206  CCATATATGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AluI          147  AGCT                 AG^CT
              215  AGCT                
HinfI           9  GAATC                G^A[ACGT]TC
              177  GATTC               
TfiI            9  GAATC                G^A[AT]TC
              177  GATTC               
TspRI          51  CACTG                CA[GC]TG[ACGT][ACGT]^
              125  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

Cac8I       MnlI