Restriction map for
pFN21AE5644
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HindIII KasI KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 123 GGTACC G^GTACC
AcsI 1123 AAATTT [GA]^AATT[CT]
AflII 243 CTTAAG C^TTAAG
AocI 535 CCTGAGG CC^T[ACGT]AGG
ApoI 1123 AAATTT [GA]^AATT[CT]
Asp700I 667 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 123 GGTACC G^GTACC
AspHI 304 GAGCAC G[AT]GC[AT]^C
BalI 631 TGGCCA TGG^CCA
BanI 123 GGTACC G^G[CT][GA]CC
BanII 1249 GAGCCC G[GA]GC[CT]^C
BbsI 316 GAAGAC GAAGAC(2/6)
BcgI 513 GCAGTATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 495 TGATCA T^GATCA
BcnI 1260 CCGGG CC^[GC]GG
BfrI 243 CTTAAG C^TTAAG
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaI 541 GAGACC GGTCTC(1/5)
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BsmI 884 GCATTC GAATGC(1/-1)
BspHI 806 TCATGA T^CATGA
BsrBI 1368 GAGCGG CCGCTC(-3/-3)
BssSI 743 CTCGTG CACGAG(-5/-1)
BstEII 619 GGTTACC G^GT[ACGT]ACC
BstF5I 585 CATCC GGATG(2/0)
BstPI 619 GGTTACC G^GT[ACGT]ACC
Bsu36I 535 CCTGAGG CC^T[ACGT]AGG
DraI 1126 TTTAAA TTT^AAA
DsaI 1412 CCGTGG C^C[GA][CT]GG
EaeI 631 TGGCCA [CT]^GGCC[GA]
Eco81I 535 CCTGAGG CC^T[ACGT]AGG
EcoO65I 619 GGTTACC G^GT[ACGT]ACC
EcoT14I 1148 CCTTGG C^C[AT][AT]GG
FauI 1370 GCGGG CCCGC(4/6)
FbaI 495 TGATCA T^GATCA
FokI 585 CATCC GGATG(9/13)
HaeII 1416 GGCGCT [GA]GCGC^[CT]
HapII 1260 CCGG C^CGG
HpaI 898 GTTAAC GTT^AAC
HpaII 1260 CCGG C^CGG
KpnI 123 GGTACC GGTAC^C
MaeII 721 ACGT A^CGT
MluNI 631 TGGCCA TGG^CCA
MscI 631 TGGCCA TGG^CCA
MslI 504 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1260 CCGG C^CGG
NciI 1260 CCGGG CC^[GC]GG
NspI 764 ACATGC [GA]CATG^[CT]
RcaI 806 TCATGA T^CATGA
SapI 1246 GAAGAGC GCTCTTC(1/4)
SspI 668 AATATT AAT^ATT
StuI 1145 AGGCCT AGG^CCT
StyI 1148 CCTTGG C^C[AT][AT]GG
XmnI 667 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 444 GCGG CCGC(-3/-1)
1370 GCGG
AfaI 124 GTAC GT^AC
711 GTAC
BseRI 118 GAGGAG GAGGAG(10/8)
538 GAGGAG
BsgI 209 CTGCAC GTGCAG(16/14)
511 GTGCAG
BsmAI 25 GAGAC GTCTC(1/5)
541 GAGAC
CfoI 816 GCGC GCG^C
1417 GCGC
DraII 1110 AGGCCCT [GA]G^G[ACGT]CC[CT]
1222 GGGGCCT
EarI 1018 GAAGAG CTCTTC(1/4)
1246 GAAGAG
EcoNI 655 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1269 CCTGACCCAGG
EcoO109I 1110 AGGCCCT [GA]G^G[ACGT]CC[CT]
1222 GGGGCCT
HhaI 816 GCGC GCG^C
1417 GCGC
HinP1I 816 GCGC G^CGC
1417 GCGC
HincII 760 GTCAAC GT[CT]^[GA]AC
898 GTTAAC
HindII 760 GTCAAC GT[CT]^[GA]AC
898 GTTAAC
HphI 160 GGTGA GGTGA(8/7)
239 TCACC
Ksp632I 1018 GAAGAG CTCTTC(1/4)
1246 GAAGAG
PleI 236 GACTC GAGTC(4/5)
1076 GAGTC
PstI 828 CTGCAG CTGCA^G
1378 CTGCAG
RsaI 124 GTAC GT^AC
711 GTAC
SfcI 828 CTGCAG C^T[GA][CT]AG
1378 CTGCAG
Tsp45I 718 GTGAC ^GT[GC]AC
1316 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AluI AlwI AlwNI AvaII BamHI
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PflMI Sau3AI
Sau96I ScrFI SmlI TfiI Tru9I
TseI Tsp509I TspRI Van91I XhoII