Restriction map for
pFN21AE5645
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HapII
HgaI HindIII HpaI HpaII KpnI
KspI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 1239 GGCGCC G[GA]^CG[CT]C
AseI 856 ATTAAT AT^TAAT
AsnI 856 ATTAAT AT^TAAT
Asp700I 1384 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 223 CTCGAG C^[CT]CG[GA]G
AvaII 297 GGTCC G^G[AT]CC
BalI 556 TGGCCA TGG^CCA
BamHI 997 GGATCC G^GATCC
BanI 1239 GGCGCC G^G[CT][GA]CC
BbeI 1239 GGCGCC GGCGC^C
BcgI 744 CGAACAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1058 TGATCA T^GATCA
BlpI 724 GCTGAGC GC^T[ACGT]AGC
BpmI 337 CTGGAG CTGGAG(16/14)
Bpu1102I 724 GCTGAGC GC^T[ACGT]AGC
BsaBI 238 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1239 GGCGCC G[GA]^CG[CT]C
BsgI 83 CTGCAC GTGCAG(16/14)
BsmAI 1341 GAGAC GTCTC(1/5)
BsmBI 1341 GAGACG CGTCTC(1/5)
BsmFI 298 GTCCC GGGAC(10/14)
BsoBI 223 CTCGAG C^[CT]CG[GA]G
BstBI 542 TTCGAA TT^CGAA
BstYI 997 GGATCC [GA]^GATC[CT]
CelII 724 GCTGAGC GC^T[ACGT]AGC
CfoI 1240 GCGC GCG^C
DraII 227 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 964 GACATAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 556 TGGCCA [CT]^GGCC[GA]
EcoO109I 227 AGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 1058 TGATCA T^GATCA
HaeII 1239 GGCGCC [GA]GCGC^[CT]
HhaI 1240 GCGC GCG^C
Hin1I 1239 GGCGCC G[GA]^CG[CT]C
HinP1I 1240 GCGC G^CGC
HincII 385 GTCAAC GT[CT]^[GA]AC
HindII 385 GTCAAC GT[CT]^[GA]AC
KasI 1239 GGCGCC G^GCGCC
MamI 238 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 997 GGATCC [GA]^GATC[CT]
MluNI 556 TGGCCA TGG^CCA
MscI 556 TGGCCA TGG^CCA
NarI 1239 GGCGCC GG^CGCC
NspI 389 ACATGC [GA]CATG^[CT]
NspV 542 TTCGAA TT^CGAA
PaeR7I 223 CTCGAG C^TCGAG
PshBI 856 ATTAAT AT^TAAT
PstI 1401 CTGCAG CTGCA^G
SapI 1384 GAAGAGC GCTCTTC(1/4)
SexAI 293 ACCTGGT A^CC[AT]GGT
SfcI 1401 CTGCAG C^T[GA][CT]AG
SfoI 1239 GGCGCC GGC^GCC
SfuI 542 TTCGAA TT^CGAA
XbaI 1303 TCTAGA T^CTAGA
XcmI 759 CCAAGTGAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 223 CTCGAG C^TCGAG
XhoII 997 GGATCC [GA]^GATC[CT]
XmnI 1384 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 998 GATCC GGATC(4/5)
997 GGATC
BanII 1015 GAGCCC G[GA]GC[CT]^C
1141 GAGCCC
BbvI 1403 GCAGC GCAGC(8/12)
141 GCTGC
BfaI 685 CTAG C^TAG
1304 CTAG
Bsp1407I 303 TGTACA T^GTACA
317 TGTACA
BsrGI 303 TGTACA T^GTACA
317 TGTACA
Cac8I 1242 GCCCGC GC[ACGT]^[ACGT]GC
1274 GCAAGC
DsaI 843 CCATGG C^C[GA][CT]GG
1145 CCATGG
EcoT14I 843 CCATGG C^C[AT][AT]GG
1145 CCATGG
FauI 1243 CCCGC CCCGC(4/6)
1259 GCGGG
MaeI 685 CTAG C^TAG
1304 CTAG
MaeII 1007 ACGT A^CGT
1344 ACGT
MaeIII 668 GTGAC ^GT[ACGT]AC
1358 GTGAC
MslI 619 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
838 CACAACCATG
MwoI 17 GCGACCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
715 GCTCAAAAGGC
NcoI 843 CCATGG C^CATGG
1145 CCATGG
SspBI 303 TGTACA T^GTACA
317 TGTACA
StyI 843 CCATGG C^C[AT][AT]GG
1145 CCATGG
TaqI 224 TCGA T^CGA
543 TCGA
TseI 141 GCTGC G^C[AT]GC
1403 GCAGC
Tsp45I 668 GTGAC ^GT[GC]AC
1358 GTGAC
TthHB8I 224 TCGA T^CGA
543 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AspHI BbsI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TfiI Tru9I Tsp509I TspRI