##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          235  GTAGAC               GT^[AC][GT]AC
AlwI           27  GATCC                GGATC(4/5)
AocI           78  CCTCAGG              CC^T[ACGT]AGG
BbvCI          36  CCTCAGC              CCTCAGC(-5/-2)
BmyI          215  GGGCAC               G[AGT]GC[ACT]^C
BpmI          160  CTCCAG               CTGGAG(16/14)
BsaWI         122  ACCGGA               [AT]^CCGG[AT]
BseRI         206  GAGGAG               GAGGAG(10/8)
Bsp1286I      215  GGGCAC               G[AGT]GC[ACT]^C
BspHI         170  TCATGA               T^CATGA
Bsu36I         78  CCTCAGG              CC^T[ACGT]AGG
DpnII          27  GATC                 ^GATC
Eco81I         78  CCTCAGG              CC^T[ACGT]AGG
FauI          149  CCCGC                CCCGC(4/6)
HapII         123  CCGG                 C^CGG
HpaII         123  CCGG                 C^CGG
MboI           27  GATC                 ^GATC
MslI          279  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        139  CAGCTG               C[AC]G^C[GT]G
MspI          123  CCGG                 C^CGG
NdeII          27  GATC                 ^GATC
NspI          242  GCATGT               [GA]CATG^[CT]
PstI          295  CTGCAG               CTGCA^G
PvuII         139  CAGCTG               CAG^CTG
RcaI          170  TCATGA               T^CATGA
Sau3AI         27  GATC                 ^GATC
SfcI          295  CTGCAG               C^T[GA][CT]AG
Tsp509I       269  AATT                 ^AATT
TspRI          52  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsaJI         314  CCCAGG               C^C[ACGT][ACGT]GG
              315  CCAGGG              
BstF5I        279  CATCC                GGATG(2/0)
               83  GGATG               
Cfr13I        135  GGGCC                G^G[ACGT]CC
              288  GGCCC               
DdeI           37  CTCAG                C^T[ACGT]AG
               79  CTCAG               
FokI          279  CATCC                GGATG(9/13)
               83  GGATG               
HaeIII        136  GGCC                 GG^CC
              288  GGCC                
HphI           32  TCACC                GGTGA(8/7)
              110  TCACC               
MboII          17  TCTTC                GAAGA(8/7)
              107  TCTTC               
NlaIII        171  CATG                 CATG^
              243  CATG                
Sau96I        135  GGGCC                G^G[ACGT]CC
              288  GGCCC               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        AluI        BbvI        BssKI       BstNI       
Cac8I       EcoRII      Fnu4HI      ItaI        MnlI        
MvaI        MwoI        ScrFI       SfaNI       TseI