Restriction map for
pFN21AE5440
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbvCI BcgI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI EarI
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 94 GTAGAC GT^[AC][GT]AC
AfaI 708 GTAC GT^AC
AflII 75 CTTAAG C^TTAAG
AflIII 85 ACGTGT A^C[GA][CT]GT
AhdI 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 910 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 941 AGCGCT AGC^GCT
AspEI 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 366 GGATCC G^GATCC
BbrPI 84 CACGTG CAC^GTG
BciVI 900 GGATAC GTATCC(6/5)
BclI 830 TGATCA T^GATCA
BcnI 857 CCCGG CC^[GC]GG
BfaI 721 CTAG C^TAG
BfrI 75 CTTAAG C^TTAAG
BglII 346 AGATCT A^GATCT
BsaAI 84 CACGTG [CT]AC^GT[GA]
BseRI 577 GAGGAG GAGGAG(10/8)
BsmAI 144 GTCTC GTCTC(1/5)
BsmFI 104 GGGAC GGGAC(10/14)
Bsp1407I 707 TGTACA T^GTACA
BspMI 386 ACCTGC ACCTGC(4/8)
BsrBI 967 GAGCGG CCGCTC(-3/-3)
BsrFI 562 GCCGGC [GA]^CCGG[CT]
BsrGI 707 TGTACA T^GTACA
BstAPI 668 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 716 CCATCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 942 GCGC GCG^C
Cfr10I 562 GCCGGC [GA]^CCGG[CT]
DraII 581 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 941 AGCGCT AGC^GCT
EcoO109I 581 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 776 GAATTC G^AATTC
FbaI 830 TGATCA T^GATCA
HaeII 941 AGCGCT [GA]GCGC^[CT]
HhaI 942 GCGC GCG^C
HinP1I 942 GCGC G^CGC
MaeI 721 CTAG C^TAG
MaeIII 960 GTTAC ^GT[ACGT]AC
MseI 76 TTAA T^TAA
MslI 717 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 562 GCCGGC GCC^GGC
NciI 857 CCCGG CC^[GC]GG
NgoMIV 562 GCCGGC G^CCGGC
NspI 956 GCATGT [GA]CATG^[CT]
PmaCI 84 CACGTG CAC^GTG
PmlI 84 CACGTG CAC^GTG
RsaI 708 GTAC GT^AC
SspBI 707 TGTACA T^GTACA
StuI 838 AGGCCT AGG^CCT
Tru9I 76 TTAA T^TAA
XcmI 716 CCATCCTAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 760 GAATTT [GA]^AATT[CT]
776 GAATTC
AocI 361 CCTGAGG CC^T[ACGT]AGG
841 CCTAAGG
ApoI 760 GAATTT [GA]^AATT[CT]
776 GAATTC
BalI 392 TGGCCA TGG^CCA
483 TGGCCA
BbsI 111 GAAGAC GAAGAC(2/6)
606 GAAGAC
BpuAI 111 GAAGAC GAAGAC(2/6)
606 GAAGAC
BsgI 539 CTGCAC GTGCAG(16/14)
590 CTGCAC
BsrI 246 CCAGT ACTGG(1/-1)
486 CCAGT
Bsu36I 361 CCTGAGG CC^T[ACGT]AGG
841 CCTAAGG
Eco81I 361 CCTGAGG CC^T[ACGT]AGG
841 CCTAAGG
FauI 298 CCCGC CCCGC(4/6)
969 GCGGG
HapII 563 CCGG C^CGG
858 CCGG
HpaII 563 CCGG C^CGG
858 CCGG
MaeII 85 ACGT A^CGT
98 ACGT
MluNI 392 TGGCCA TGG^CCA
483 TGGCCA
MscI 392 TGGCCA TGG^CCA
483 TGGCCA
MspI 563 CCGG C^CGG
858 CCGG
PleI 509 GACTC GAGTC(4/5)
46 GAGTC
PstI 271 CTGCAG CTGCA^G
766 CTGCAG
SfaNI 161 GCATC GCATC(5/9)
448 GCATC
SfcI 271 CTGCAG C^T[GA][CT]AG
766 CTGCAG
SmlI 75 CTTAAG C^T[CT][GA]AG
755 CTCAAG
TaqI 216 TCGA T^CGA
252 TCGA
TthHB8I 216 TCGA T^CGA
252 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TfiI TseI Tsp509I TspRI XhoII