Restriction map for
pFN21AE5578
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BbrPI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BspDI
BspHI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI HincII HindII HindIII
HpaI MaeII MamI MfeI MluI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SbfI ScaI SfcI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tru9I Tsp509I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 953 GGTACC G^GTACC
AccIII 481 TCCGGA T^CCGGA
AflIII 473 ACATGT A^C[GA][CT]GT
AocI 1042 CCTGAGG CC^T[ACGT]AGG
ApaI 41 GGGCCC GGGCC^C
Asp718I 953 GGTACC G^GTACC
AvaII 125 GGTCC G^G[AT]CC
AviII 386 TGCGCA TGC^GCA
BalI 234 TGGCCA TGG^CCA
BamHI 732 GGATCC G^GATCC
BanII 41 GGGCCC G[GA]GC[CT]^C
BbsI 1358 GTCTTC GAAGAC(2/6)
BclI 998 TGATCA T^GATCA
BfaI 79 CTAG C^TAG
BglII 461 AGATCT A^GATCT
BpmI 753 CTCCAG CTGGAG(16/14)
BpuAI 1358 GTCTTC GAAGAC(2/6)
BsaWI 481 TCCGGA [AT]^CCGG[AT]
BseAI 481 TCCGGA T^CCGGA
BsiEI 402 CGGCCG CG[GA][CT]^CG
BsmBI 855 CGTCTC CGTCTC(1/5)
BspEI 481 TCCGGA T^CCGGA
BspLU11I 473 ACATGT A^CATGT
BsrDI 84 CATTGC GCAATG(2/0)
BsrFI 195 GCCGGT [GA]^CCGG[CT]
BssSI 661 CACGAG CACGAG(-5/-1)
Bsu36I 1042 CCTGAGG CC^T[ACGT]AGG
Cfr10I 195 GCCGGT [GA]^CCGG[CT]
DrdI 73 GACATCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 402 CGGCCG C^GGCCG
EclXI 402 CGGCCG C^GGCCG
Eco52I 402 CGGCCG C^GGCCG
Eco81I 1042 CCTGAGG CC^T[ACGT]AGG
FbaI 998 TGATCA T^GATCA
FspI 386 TGCGCA TGC^GCA
KpnI 953 GGTACC GGTAC^C
KspI 1099 CCGCGG CCGC^GG
MaeI 79 CTAG C^TAG
MluNI 234 TGGCCA TGG^CCA
MroI 481 TCCGGA T^CCGGA
MscI 234 TGGCCA TGG^CCA
NotI 401 GCGGCCGC GC^GGCCGC
NspI 473 ACATGT [GA]CATG^[CT]
PshAI 612 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1207 CAGCTG CAG^CTG
SacII 1099 CCGCGG CCGC^GG
SexAI 881 ACCAGGT A^CC[AT]GGT
SfiI 42 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Alw44I 1250 GTGCAC G^TGCAC
1318 GTGCAC
Aor51HI 392 AGCGCT AGC^GCT
896 AGCGCT
ApaLI 1250 GTGCAC G^TGCAC
1318 GTGCAC
AspI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
BbeI 631 GGCGCC GGCGC^C
1130 GGCGCC
BglI 43 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1122 GCCCAACGGGC
BsaI 682 GGTCTC GGTCTC(1/5)
1309 GGTCTC
BsmFI 256 GGGAC GGGAC(10/14)
1141 GGGAC
BsmI 300 GAATGC GAATGC(1/-1)
1007 GCATTC
Bsp1407I 446 TGTACA T^GTACA
593 TGTACA
BspMI 383 ACCTGC ACCTGC(4/8)
791 ACCTGC
BsrGI 446 TGTACA T^GTACA
593 TGTACA
DdeI 465 CTCAG C^T[ACGT]AG
1043 CTGAG
Eco47III 392 AGCGCT AGC^GCT
896 AGCGCT
HgaI 176 GACGC GACGC(5/10)
966 GACGC
KasI 631 GGCGCC G^GCGCC
1130 GGCGCC
NarI 631 GGCGCC GG^CGCC
1130 GGCGCC
PflFI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
SapI 912 GAAGAGC GCTCTTC(1/4)
306 GCTCTTC
SfoI 631 GGCGCC GGC^GCC
1130 GGCGCC
SspBI 446 TGTACA T^GTACA
593 TGTACA
TfiI 290 GATTC G^A[AT]TC
1180 GATTC
Tsp45I 568 GTGAC ^GT[GC]AC
808 GTGAC
Tth111I 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
XcmI 458 CCAAGATCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
971 CCATGTTTCTCATGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI BanI
BbvI BcgI BcnI BmyI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I Van91I XhoII