Restriction map for
pFN21AE5548
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BspDI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tth111I TthHB8I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflIII 527 ACATGT A^C[GA][CT]GT
AhdI 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 106 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 347 CCCGAG C^[CT]CG[GA]G
AvaII 1065 GGTCC G^G[AT]CC
BalI 837 TGGCCA TGG^CCA
BanII 559 GAGCTC G[GA]GC[CT]^C
BciVI 92 GTATCC GTATCC(6/5)
BclI 543 TGATCA T^GATCA
BfaI 1138 CTAG C^TAG
BglII 446 AGATCT A^GATCT
BlpI 934 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 934 GCTCAGC GC^T[ACGT]AGC
BsaI 509 GGTCTC GGTCTC(1/5)
BsgI 308 GTGCAG GTGCAG(16/14)
BsmI 100 GAATGC GAATGC(1/-1)
BsoBI 347 CCCGAG C^[CT]CG[GA]G
Bsp1407I 941 TGTACA T^GTACA
BspLU11I 527 ACATGT A^CATGT
BspMI 902 ACCTGC ACCTGC(4/8)
BsrDI 605 GCAATG GCAATG(2/0)
BsrGI 941 TGTACA T^GTACA
BssSI 1082 CACGAG CACGAG(-5/-1)
BstEII 255 GGTCACC G^GT[ACGT]ACC
BstPI 255 GGTCACC G^GT[ACGT]ACC
Cac8I 687 GCTGGC GC[ACGT]^[ACGT]GC
CelII 934 GCTCAGC GC^T[ACGT]AGC
CfoI 567 GCGC GCG^C
DraII 693 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 702 CCATGG C^C[GA][CT]GG
EaeI 837 TGGCCA [CT]^GGCC[GA]
Eam1105I 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 144 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 693 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 255 GGTCACC G^GT[ACGT]ACC
FbaI 543 TGATCA T^GATCA
HhaI 567 GCGC GCG^C
HinP1I 567 GCGC G^CGC
MaeI 1138 CTAG C^TAG
MluNI 837 TGGCCA TGG^CCA
MscI 837 TGGCCA TGG^CCA
MseI 923 TTAA T^TAA
NcoI 702 CCATGG C^CATGG
NdeI 353 CATATG CA^TATG
NspI 527 ACATGT [GA]CATG^[CT]
PstI 745 CTGCAG CTGCA^G
SacI 559 GAGCTC GAGCT^C
SspBI 941 TGTACA T^GTACA
TfiI 987 GAATC G^A[AT]TC
Tru9I 923 TTAA T^TAA
XbaI 1137 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 566 CGCG CG^CG
866 CGCG
AciI 865 CCGC CCGC(-3/-1)
476 GCGG
AocI 148 CCTGAGG CC^T[ACGT]AGG
1124 CCTTAGG
AspHI 559 GAGCTC G[AT]GC[AT]^C
1158 GAGCAC
BbsI 400 GAAGAC GAAGAC(2/6)
855 GAAGAC
BpmI 1027 CTCCAG CTGGAG(16/14)
1113 CTGGAG
BpuAI 400 GAAGAC GAAGAC(2/6)
855 GAAGAC
BsiHKAI 559 GAGCTC G[AT]GC[AT]^C
1158 GAGCAC
BssKI 501 CCTGG ^CC[ACGT]GG
829 CCAGG
BstF5I 72 CATCC GGATG(2/0)
582 CATCC
BstNI 501 CCTGG CC^[AT]GG
829 CCAGG
BstUI 566 CGCG CG^CG
866 CGCG
BstYI 411 GGATCT [GA]^GATC[CT]
446 AGATCT
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
1124 CCTTAGG
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
1124 CCTTAGG
EcoRII 501 CCTGG ^CC[AT]GG
829 CCAGG
FokI 72 CATCC GGATG(9/13)
582 CATCC
MflI 411 GGATCT [GA]^GATC[CT]
446 AGATCT
MslI 522 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
595 CATGAGGGTG
MvaI 501 CCTGG CC^[AT]GG
829 CCAGG
MvnI 566 CGCG CG^CG
866 CGCG
NlaIV 484 GGGGCC GG[ACGT]^[ACGT]CC
1115 GGAGCC
ScaI 380 AGTACT AGT^ACT
846 AGTACT
ScrFI 501 CCTGG CC^[ACGT]GG
829 CCAGG
SfaNI 1006 GATGC GCATC(5/9)
71 GCATC
SfcI 462 CTACAG C^T[GA][CT]AG
745 CTGCAG
Tsp509I 517 AATT ^AATT
1054 AATT
XhoII 411 GGATCT [GA]^GATC[CT]
446 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BstXI Cfr13I DdeI DpnII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MwoI NdeII NlaIII PflMI
PleI PvuII RsaI Sau3AI Sau96I
StyI TseI Tsp45I TspRI Van91I
XcmI