Restriction map for
pFN21AE2731
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 791 GGTACC G^GTACC
AccI 765 GTATAC GT^[AC][GT]AC
AciI 106 CCGC CCGC(-3/-1)
AseI 930 ATTAAT AT^TAAT
AsnI 930 ATTAAT AT^TAAT
Asp718I 791 GGTACC G^GTACC
AvaII 540 GGACC G^G[AT]CC
BanI 791 GGTACC G^G[CT][GA]CC
BbsI 89 GTCTTC GAAGAC(2/6)
BciVI 863 GTATCC GTATCC(6/5)
BfaI 925 CTAG C^TAG
BpmI 338 CTGGAG CTGGAG(16/14)
BpuAI 89 GTCTTC GAAGAC(2/6)
BseRI 128 GAGGAG GAGGAG(10/8)
BsiEI 760 CGGCCG CG[GA][CT]^CG
BsiYI 211 CCTCGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 211 CCTCGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 612 GAGAC GTCTC(1/5)
BsmFI 555 GGGAC GGGAC(10/14)
Bst1107I 765 GTATAC GTA^TAC
BstZ17I 765 GTATAC GTA^TAC
EaeI 760 CGGCCG [CT]^GGCC[GA]
EagI 760 CGGCCG C^GGCCG
EarI 877 CTCTTC CTCTTC(1/4)
EclXI 760 CGGCCG C^GGCCG
Eco52I 760 CGGCCG C^GGCCG
EcoRV 274 GATATC GAT^ATC
EcoT14I 867 CCTTGG C^C[AT][AT]GG
HapII 426 CCGG C^CGG
HpaII 426 CCGG C^CGG
HphI 220 GGTGA GGTGA(8/7)
KpnI 791 GGTACC GGTAC^C
Ksp632I 877 CTCTTC CTCTTC(1/4)
MaeI 925 CTAG C^TAG
MaeII 366 ACGT A^CGT
MslI 580 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 106 CCGCTG C[AC]G^C[GT]G
MspI 426 CCGG C^CGG
MwoI 111 GCCCCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 791 GGTACC GG[ACGT]^[ACGT]CC
PleI 147 GAGTC GAGTC(4/5)
PshBI 930 ATTAAT AT^TAAT
SfaNI 484 GATGC GCATC(5/9)
SspI 40 AATATT AAT^ATT
StuI 193 AGGCCT AGG^CCT
StyI 867 CCTTGG C^C[AT][AT]GG
TfiI 565 GAATC G^A[AT]TC
Tsp45I 469 GTGAC ^GT[GC]AC
TspRI 336 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 268 AAATTT [GA]^AATT[CT]
419 AAATTC
ApoI 268 AAATTT [GA]^AATT[CT]
419 AAATTC
Asp700I 38 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
385 GAAGGCTTTC
BsaJI 323 CCAGGG C^C[ACGT][ACGT]GG
867 CCTTGG
BssKI 124 CCAGG ^CC[ACGT]GG
323 CCAGG
BstNI 124 CCAGG CC^[AT]GG
323 CCAGG
Cfr13I 208 GGGCC G^G[ACGT]CC
540 GGACC
DdeI 116 CTGAG C^T[ACGT]AG
747 CTCAG
DpnII 136 GATC ^GATC
547 GATC
EcoRII 124 CCAGG ^CC[AT]GG
323 CCAGG
HinfI 147 GAGTC G^A[ACGT]TC
565 GAATC
MaeIII 177 GTTAC ^GT[ACGT]AC
469 GTGAC
MboI 136 GATC ^GATC
547 GATC
MvaI 124 CCAGG CC^[AT]GG
323 CCAGG
NdeII 136 GATC ^GATC
547 GATC
Sau3AI 136 GATC ^GATC
547 GATC
Sau96I 208 GGGCC G^G[ACGT]CC
540 GGACC
ScrFI 124 CCAGG CC^[ACGT]GG
323 CCAGG
TaqI 408 TCGA T^CGA
735 TCGA
TthHB8I 408 TCGA T^CGA
735 TCGA
XmnI 38 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
385 GAAGGCTTTC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AfaI AluI BbvI BsrI Fnu4HI
HaeIII ItaI MboII MnlI MseI
NlaIII RsaI Tru9I TseI Tsp509I