Restriction map for
pFN21AE6381
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbsI
BbvCI BcgI BfaI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 623 GCGG CCGC(-3/-1)
AhdI 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 309 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 187 GGATCC G^GATCC
BciVI 527 GTATCC GTATCC(6/5)
BcnI 361 CCCGG CC^[GC]GG
BglII 507 AGATCT A^GATCT
BseRI 895 CTCCTC GAGGAG(10/8)
BsgI 215 GTGCAG GTGCAG(16/14)
BsmAI 535 GAGAC GTCTC(1/5)
BsmBI 535 GAGACG CGTCTC(1/5)
BstEII 709 GGTTACC G^GT[ACGT]ACC
BstPI 709 GGTTACC G^GT[ACGT]ACC
DdeI 165 CTCAG C^T[ACGT]AG
DraII 219 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 929 TGGCCG [CT]^GGCC[GA]
Eam1105I 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 823 CCTATAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 219 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 709 GGTTACC G^GT[ACGT]ACC
EcoRI 753 GAATTC G^AATTC
EcoRV 299 GATATC GAT^ATC
HapII 362 CCGG C^CGG
HinfI 72 GATTC G^A[ACGT]TC
HpaII 362 CCGG C^CGG
HphI 87 GGTGA GGTGA(8/7)
MseI 584 TTAA T^TAA
MspA1I 312 CAGCTG C[AC]G^C[GT]G
MspI 362 CCGG C^CGG
NciI 361 CCCGG CC^[GC]GG
NdeI 885 CATATG CA^TATG
NlaIII 195 CATG CATG^
PpuMI 219 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 312 CAGCTG CAG^CTG
SfaNI 626 GCATC GCATC(5/9)
SmlI 532 CTTGAG C^T[CT][GA]AG
TfiI 72 GATTC G^A[AT]TC
Tru9I 584 TTAA T^TAA
XcmI 852 CCAGACATACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 496 AAATTC [GA]^AATT[CT]
753 GAATTC
AfaI 733 GTAC GT^AC
922 GTAC
AluI 313 AGCT AG^CT
444 AGCT
AlwI 188 GATCC GGATC(4/5)
187 GGATC
ApoI 496 AAATTC [GA]^AATT[CT]
753 GAATTC
BanI 160 GGCACC G^G[CT][GA]CC
483 GGCACC
BbvI 311 GCAGC GCAGC(8/12)
394 GCTGC
BclI 305 TGATCA T^GATCA
570 TGATCA
BsaJI 41 CCCTGG C^C[ACGT][ACGT]GG
224 CCCTGG
BspMI 635 ACCTGC ACCTGC(4/8)
217 GCAGGT
BstYI 187 GGATCC [GA]^GATC[CT]
507 AGATCT
Cac8I 639 GCTTGC GC[ACGT]^[ACGT]GC
829 GCAGGC
FbaI 305 TGATCA T^GATCA
570 TGATCA
HaeIII 318 GGCC GG^CC
930 GGCC
MflI 187 GGATCC [GA]^GATC[CT]
507 AGATCT
RsaI 733 GTAC GT^AC
922 GTAC
TseI 311 GCAGC G^C[AT]GC
394 GCTGC
Tsp45I 242 GTGAC ^GT[GC]AC
343 GTGAC
XhoII 187 GGATCC [GA]^GATC[CT]
507 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AvaII BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cfr13I DpnII
EarI EcoRII Fnu4HI FokI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfcI Tsp509I