Restriction map for
pFN21AE6195
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeIII HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MspI
MunI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 8 CGCG CG^CG
AciI 15 GCGG CCGC(-3/-1)
AcsI 286 GAATTC [GA]^AATT[CT]
AhdI 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 294 GATCC GGATC(4/5)
ApoI 286 GAATTC [GA]^AATT[CT]
AspEI 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 74 CCTCAGC CCTCAGC(-5/-2)
BpmI 318 CTCCAG CTGGAG(16/14)
BsiYI 291 CCAGATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 291 CCAGATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 325 GCATTC GAATGC(1/-1)
BsrI 320 CCAGT ACTGG(1/-1)
BstUI 8 CGCG CG^CG
BstXI 38 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 293 AGATCC [GA]^GATC[CT]
DdeI 75 CTCAG C^T[ACGT]AG
DpnII 294 GATC ^GATC
Eam1105I 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 286 GAATTC G^AATTC
HaeII 48 GGCGCT [GA]GCGC^[CT]
HphI 329 TCACC GGTGA(8/7)
MaeII 68 ACGT A^CGT
MaeIII 34 GTGAC ^GT[ACGT]AC
MboI 294 GATC ^GATC
MboII 283 GAAGA GAAGA(8/7)
MflI 293 AGATCC [GA]^GATC[CT]
MslI 39 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 127 CAGCTG C[AC]G^C[GT]G
MvnI 8 CGCG CG^CG
NdeII 294 GATC ^GATC
PflMI 291 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 127 CAGCTG CAG^CTG
Sau3AI 294 GATC ^GATC
Tsp45I 34 GTGAC ^GT[GC]AC
Van91I 291 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 293 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 128 AGCT AG^CT
276 AGCT
AlwNI 77 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
292 CAGATCCTG
BbvI 4 GCAGC GCAGC(8/12)
51 GCTGC
BsaJI 38 CCATGG C^C[ACGT][ACGT]GG
350 CCATGG
BsmAI 173 GAGAC GTCTC(1/5)
28 GTCTC
BssKI 297 CCTGG ^CC[ACGT]GG
308 CCTGG
BstNI 297 CCTGG CC^[AT]GG
308 CCTGG
Cac8I 269 GCAAGC GC[ACGT]^[ACGT]GC
273 GCAAGC
CfoI 7 GCGC GCG^C
49 GCGC
DsaI 38 CCATGG C^C[GA][CT]GG
350 CCATGG
EcoRII 297 CCTGG ^CC[AT]GG
308 CCTGG
EcoT14I 38 CCATGG C^C[AT][AT]GG
350 CCATGG
Fnu4HI 4 GCAGC GC^[ACGT]GC
51 GCTGC
HhaI 7 GCGC GCG^C
49 GCGC
HinP1I 7 GCGC G^CGC
49 GCGC
ItaI 4 GCAGC GC^[ACGT]GC
51 GCTGC
MnlI 74 CCTC CCTC(7/6)
80 CCTC
MvaI 297 CCTGG CC^[AT]GG
308 CCTGG
NcoI 38 CCATGG C^CATGG
350 CCATGG
ScrFI 297 CCTGG CC^[ACGT]GG
308 CCTGG
StyI 38 CCATGG C^C[AT][AT]GG
350 CCATGG
TseI 4 GCAGC G^C[AT]GC
51 GCTGC
Tsp509I 211 AATT ^AATT
287 AATT
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
NlaIII TspRI