##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaI        BsaJI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       Cfr10I      Cfr13I      ClaI        CpoI        
DpnII       DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeIII      
HapII       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       ItaI        KpnI        KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        NaeI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         154  CGCG                 CG^CG
AcyI          116  GGCGCC               G[GA]^CG[CT]C
BanI          116  GGCGCC               G^G[CT][GA]CC
BanII         204  GGGCTC               G[GA]GC[CT]^C
BbeI          116  GGCGCC               GGCGC^C
BlpI          174  GCTGAGC              GC^T[ACGT]AGC
BmyI          204  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I      174  GCTGAGC              GC^T[ACGT]AGC
BsaHI         116  GGCGCC               G[GA]^CG[CT]C
BseRI           7  GAGGAG               GAGGAG(10/8)
BsgI          124  CTGCAC               GTGCAG(16/14)
BsiYI          96  CCATGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           96  CCATGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          40  GTCTC                GTCTC(1/5)
Bsp1286I      204  GGGCTC               G[AGT]GC[ACT]^C
BsrDI         143  CATTGC               GCAATG(2/0)
BssKI          31  CCTGG                ^CC[ACGT]GG
BstNI          31  CCTGG                CC^[AT]GG
BstUI         154  CGCG                 CG^CG
CelII         174  GCTGAGC              GC^T[ACGT]AGC
DraI           49  TTTAAA               TTT^AAA
DraIII        127  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI            4  GAAGAG               CTCTTC(1/4)
EcoRII         31  CCTGG                ^CC[AT]GG
HaeII         116  GGCGCC               [GA]GCGC^[CT]
HgaI          155  GCGTC                GACGC(5/10)
Hin1I         116  GGCGCC               G[GA]^CG[CT]C
KasI          116  GGCGCC               G^GCGCC
Ksp632I         4  GAAGAG               CTCTTC(1/4)
MaeIII        106  GTGAC                ^GT[ACGT]AC
MboII           4  GAAGA                GAAGA(8/7)
MseI           50  TTAA                 T^TAA
MslI          129  CACTGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           31  CCTGG                CC^[AT]GG
MvnI          154  CGCG                 CG^CG
NarI          116  GGCGCC               GG^CGCC
NlaIII         97  CATG                 CATG^
ScrFI          31  CCTGG                CC^[ACGT]GG
SfoI          116  GGCGCC               GGC^GCC
TaqI          158  TCGA                 T^CGA
Tru9I          50  TTAA                 T^TAA
Tsp45I        106  GTGAC                ^GT[GC]AC
Tsp509I        36  AATT                 ^AATT
TspRI         129  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       158  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

CfoI          117  GCGC                 GCG^C
              153  GCGC                
DdeI          149  CTGAG                C^T[ACGT]AG
              175  CTGAG               
HhaI          117  GCGC                 GCG^C
              153  GCGC                
HinP1I        117  GCGC                 G^CGC
              153  GCGC                
HphI           70  GGTGA                GGTGA(8/7)
              105  GGTGA               
NlaIV         116  GGCGCC               GG[ACGT]^[ACGT]CC
              205  GGCTCC              


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MnlI        MwoI