Restriction map for
pFN21AE5190
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI AlwNI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Cac8I
CelII CfoI Cfr10I CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboII MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI TseI Tsp45I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 37 GTATAC GT^[AC][GT]AC
Alw44I 48 GTGCAC G^TGCAC
AocI 42 CCTGAGG CC^T[ACGT]AGG
ApaLI 48 GTGCAC G^TGCAC
AspHI 48 GTGCAC G[AT]GC[AT]^C
BclI 140 TGATCA T^GATCA
BmyI 48 GTGCAC G[AGT]GC[ACT]^C
BsaWI 52 ACCGGA [AT]^CCGG[AT]
BsiHKAI 48 GTGCAC G[AT]GC[AT]^C
BsmAI 170 GAGAC GTCTC(1/5)
BsmFI 301 GGGAC GGGAC(10/14)
BsmI 382 GAATGC GAATGC(1/-1)
Bsp1286I 48 GTGCAC G[AGT]GC[ACT]^C
BspDI 355 ATCGAT AT^CGAT
BspHI 345 TCATGA T^CATGA
BsrDI 234 CATTGC GCAATG(2/0)
Bst1107I 37 GTATAC GTA^TAC
BstF5I 340 CATCC GGATG(2/0)
BstYI 293 AGATCC [GA]^GATC[CT]
BstZ17I 37 GTATAC GTA^TAC
Bsu36I 42 CCTGAGG CC^T[ACGT]AGG
ClaI 355 ATCGAT AT^CGAT
DrdI 303 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 333 TGGCCG [CT]^GGCC[GA]
Eco81I 42 CCTGAGG CC^T[ACGT]AGG
FbaI 140 TGATCA T^GATCA
FokI 340 CATCC GGATG(9/13)
HapII 53 CCGG C^CGG
HincII 229 GTCAAC GT[CT]^[GA]AC
HindII 229 GTCAAC GT[CT]^[GA]AC
HpaII 53 CCGG C^CGG
HphI 58 GGTGA GGTGA(8/7)
MflI 293 AGATCC [GA]^GATC[CT]
MslI 340 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 53 CCGG C^CGG
MwoI 70 GCCACTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 346 CATG CATG^
PflMI 309 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 345 TCATGA T^CATGA
SfaNI 246 GATGC GCATC(5/9)
SmlI 154 CTCAAG C^T[CT][GA]AG
Van91I 309 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 71 CCACTTCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 293 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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AlwI 257 GATCC GGATC(4/5)
294 GATCC
BsiYI 88 CCCAAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
309 CCAGTCAGTGG
BslI 88 CCCAAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
309 CCAGTCAGTGG
BsrI 309 CCAGT ACTGG(1/-1)
392 CCAGT
Cfr13I 84 GGCCC G^G[ACGT]CC
97 GGCCC
DdeI 43 CTGAG C^T[ACGT]AG
261 CTTAG
MseI 194 TTAA T^TAA
287 TTAA
Sau96I 84 GGCCC G^G[ACGT]CC
97 GGCCC
Tru9I 194 TTAA T^TAA
287 TTAA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AciI BanI BsaJI BssKI BstNI
DpnII EcoRII Fnu4HI HaeIII ItaI
MboI MnlI MvaI NdeII NlaIV
Sau3AI ScrFI TaqI TspRI TthHB8I