Restriction map for
pFN21AE5294
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaI BsiEI
BsiWI BsmFI BsmI BspDI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CfoI ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI HinP1I KasI KpnI Ksp632I
KspI MaeIII MboI MflI MluI
MluNI MscI MspA1I MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 397 GACGTC GACGT^C
AccIII 248 TCCGGA T^CCGGA
AcyI 397 GACGTC G[GA]^CG[CT]C
AfaI 72 GTAC GT^AC
AlwNI 593 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 227 CTCGGG C^[CT]CG[GA]G
BanI 212 GGCACC G^G[CT][GA]CC
BanII 292 GAGCTC G[GA]GC[CT]^C
BbsI 439 GAAGAC GAAGAC(2/6)
BcnI 158 CCGGG CC^[GC]GG
BfaI 388 CTAG C^TAG
BlpI 186 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
BpuAI 439 GAAGAC GAAGAC(2/6)
BsaBI 334 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 397 GACGTC G[GA]^CG[CT]C
BseAI 248 TCCGGA T^CCGGA
BseRI 418 GAGGAG GAGGAG(10/8)
BsgI 514 GTGCAG GTGCAG(16/14)
BsiYI 152 CCACCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 152 CCACCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 395 GAGAC GTCTC(1/5)
BsmBI 395 GAGACG CGTCTC(1/5)
BsoBI 227 CTCGGG C^[CT]CG[GA]G
Bsp1407I 71 TGTACA T^GTACA
BspEI 248 TCCGGA T^CCGGA
BspHI 330 TCATGA T^CATGA
BspMI 619 GCAGGT ACCTGC(4/8)
BsrDI 614 GCAATG GCAATG(2/0)
BsrFI 256 ACCGGC [GA]^CCGG[CT]
BsrGI 71 TGTACA T^GTACA
BsrI 527 ACTGG ACTGG(1/-1)
BssSI 45 CTCGTG CACGAG(-5/-1)
BstF5I 75 CATCC GGATG(2/0)
BstXI 368 CCACTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 186 GCTGAGC GC^T[ACGT]AGC
Cfr10I 256 ACCGGC [GA]^CCGG[CT]
DraII 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 269 TGGCCG [CT]^GGCC[GA]
EcoO109I 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 232 GAATTC G^AATTC
FauI 275 CCCGC CCCGC(4/6)
FokI 75 CATCC GGATG(9/13)
Hin1I 397 GACGTC G[GA]^CG[CT]C
HindIII 382 AAGCTT A^AGCTT
HpaI 588 GTTAAC GTT^AAC
MaeI 388 CTAG C^TAG
MaeII 398 ACGT A^CGT
MamI 334 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 434 CAATTG C^AATTG
MroI 248 TCCGGA T^CCGGA
MseI 589 TTAA T^TAA
MunI 434 CAATTG C^AATTG
NciI 158 CCGGG CC^[GC]GG
NspI 164 ACATGC [GA]CATG^[CT]
RcaI 330 TCATGA T^CATGA
RsaI 72 GTAC GT^AC
SacI 292 GAGCTC GAGCT^C
SspBI 71 TGTACA T^GTACA
TaqI 4 TCGA T^CGA
Tru9I 589 TTAA T^TAA
TthHB8I 4 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 189 GAGCAC G[AT]GC[AT]^C
292 GAGCTC
BmyI 189 GAGCAC G[AGT]GC[ACT]^C
292 GAGCTC
BsaWI 248 TCCGGA [AT]^CCGG[AT]
449 ACCGGA
BsiHKAI 189 GAGCAC G[AT]GC[AT]^C
292 GAGCTC
Bsp1286I 189 GAGCAC G[AGT]GC[ACT]^C
292 GAGCTC
BstNI 59 CCAGG CC^[AT]GG
78 CCAGG
Cfr13I 56 GGGCC G^G[ACGT]CC
243 GGCCC
EcoRII 59 CCAGG ^CC[AT]GG
78 CCAGG
EcoT14I 194 CCTTGG C^C[AT][AT]GG
216 CCTTGG
HincII 429 GTTGAC GT[CT]^[GA]AC
588 GTTAAC
HindII 429 GTTGAC GT[CT]^[GA]AC
588 GTTAAC
HphI 63 GGTGA GGTGA(8/7)
123 GGTGA
MslI 75 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
369 CACTCTGGTG
MvaI 59 CCAGG CC^[AT]GG
78 CCAGG
MwoI 596 GCTCTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
605 GCTCAGAAAGC
NlaIV 84 GGAGCC GG[ACGT]^[ACGT]CC
212 GGCACC
PleI 225 GACTC GAGTC(4/5)
206 GAGTC
PstI 116 CTGCAG CTGCA^G
461 CTGCAG
Sau96I 56 GGGCC G^G[ACGT]CC
243 GGCCC
SfcI 116 CTGCAG C^T[GA][CT]AG
461 CTGCAG
StyI 194 CCTTGG C^C[AT][AT]GG
216 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BssKI Cac8I DdeI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MboII MnlI MspI NlaIII ScrFI
TfiI TseI Tsp509I