Restriction map for
pFN21AE6192
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 61 GTAC GT^AC
AlwI 117 GGATC GGATC(4/5)
AspHI 65 GAGCTC G[AT]GC[AT]^C
BanII 65 GAGCTC G[GA]GC[CT]^C
BbsI 91 GAAGAC GAAGAC(2/6)
BbvI 212 GCAGC GCAGC(8/12)
BfaI 25 CTAG C^TAG
BglI 23 GCCTAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 65 GAGCTC G[AGT]GC[ACT]^C
BpmI 207 CTGGAG CTGGAG(16/14)
BpuAI 91 GAAGAC GAAGAC(2/6)
BsiHKAI 65 GAGCTC G[AT]GC[AT]^C
Bsp1286I 65 GAGCTC G[AGT]GC[ACT]^C
BspHI 69 TCATGA T^CATGA
BstF5I 225 GGATG GGATG(2/0)
Cfr13I 147 GGCCC G^G[ACGT]CC
DpnII 118 GATC ^GATC
DsaI 221 CCATGG C^C[GA][CT]GG
EarI 84 GAAGAG CTCTTC(1/4)
EcoT14I 221 CCATGG C^C[AT][AT]GG
Fnu4HI 212 GCAGC GC^[ACGT]GC
FokI 225 GGATG GGATG(9/13)
HapII 139 CCGG C^CGG
HpaII 139 CCGG C^CGG
ItaI 212 GCAGC GC^[ACGT]GC
Ksp632I 84 GAAGAG CTCTTC(1/4)
MaeI 25 CTAG C^TAG
MaeIII 125 GTGAC ^GT[ACGT]AC
MboI 118 GATC ^GATC
MspI 139 CCGG C^CGG
MwoI 23 GCCTAGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 221 CCATGG C^CATGG
NdeII 118 GATC ^GATC
RcaI 69 TCATGA T^CATGA
RsaI 61 GTAC GT^AC
SacI 65 GAGCTC GAGCT^C
SapI 84 GAAGAGC GCTCTTC(1/4)
Sau3AI 118 GATC ^GATC
Sau96I 147 GGCCC G^G[ACGT]CC
StuI 200 AGGCCT AGG^CCT
StyI 221 CCATGG C^C[AT][AT]GG
TaqI 236 TCGA T^CGA
TseI 212 GCAGC G^C[AT]GC
Tsp45I 125 GTGAC ^GT[GC]AC
Tsp509I 42 AATT ^AATT
TspRI 182 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 236 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 66 AGCT AG^CT
108 AGCT
BsaJI 11 CCAGGG C^C[ACGT][ACGT]GG
221 CCATGG
BsiYI 132 CCCCTTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
216 CCTCTCCATGG
BslI 132 CCCCTTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
216 CCTCTCCATGG
BssKI 11 CCAGG ^CC[ACGT]GG
206 CCTGG
BstNI 11 CCAGG CC^[AT]GG
206 CCTGG
EcoRII 11 CCAGG ^CC[AT]GG
206 CCTGG
HaeIII 147 GGCC GG^CC
201 GGCC
MboII 84 GAAGA GAAGA(8/7)
91 GAAGA
MslI 48 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
70 CATGAAAGTG
MvaI 11 CCAGG CC^[AT]GG
206 CCTGG
ScrFI 11 CCAGG CC^[ACGT]GG
206 CCTGG
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
Cac8I MnlI NlaIII