Restriction map for
pFN21AE6293
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 474 GCGG CCGC(-3/-1)
AgeI 172 ACCGGT A^CCGGT
ApaI 708 GGGCCC GGGCC^C
AspHI 592 GAGCAC G[AT]GC[AT]^C
BamHI 253 GGATCC G^GATCC
BanI 492 GGTGCC G^G[CT][GA]CC
BcgI 353 GCAGCAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 533 AGATCT A^GATCT
BsaI 677 GGTCTC GGTCTC(1/5)
BsaWI 172 ACCGGT [AT]^CCGG[AT]
BsgI 413 GTGCAG GTGCAG(16/14)
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
BsrFI 172 ACCGGT [GA]^CCGG[CT]
Cfr10I 172 ACCGGT [GA]^CCGG[CT]
DdeI 447 CTTAG C^T[ACGT]AG
EcoRI 178 GAATTC G^AATTC
FauI 474 GCGGG CCCGC(4/6)
HapII 173 CCGG C^CGG
HgaI 39 GACGC GACGC(5/10)
HincII 115 GTCAAC GT[CT]^[GA]AC
HindII 115 GTCAAC GT[CT]^[GA]AC
HpaII 173 CCGG C^CGG
MaeIII 217 GTAAC ^GT[ACGT]AC
MseI 343 TTAA T^TAA
MslI 519 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 173 CCGG C^CGG
PinAI 172 ACCGGT A^CCGGT
PleI 359 GAGTC GAGTC(4/5)
PstI 668 CTGCAG CTGCA^G
SapI 501 GCTCTTC GCTCTTC(1/4)
SfcI 668 CTGCAG C^T[GA][CT]AG
TfiI 549 GAATC G^A[AT]TC
Tru9I 343 TTAA T^TAA
XcmI 392 CCAGACTTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AcsI 178 GAATTC [GA]^AATT[CT]
283 AAATTT
AfaI 99 GTAC GT^AC
444 GTAC
ApoI 178 GAATTC [GA]^AATT[CT]
283 AAATTT
AvaII 170 GGACC G^G[AT]CC
435 GGTCC
BanII 479 GGGCTC G[GA]GC[CT]^C
708 GGGCCC
BbsI 187 GAAGAC GAAGAC(2/6)
542 GTCTTC
BpuAI 187 GAAGAC GAAGAC(2/6)
542 GTCTTC
BsiYI 235 CCATTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
471 CCTGCGGGGGG
BslI 235 CCATTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
471 CCTGCGGGGGG
BsmAI 154 GAGAC GTCTC(1/5)
678 GTCTC
BsmFI 436 GTCCC GGGAC(10/14)
719 GTCCC
BspMI 470 ACCTGC ACCTGC(4/8)
415 GCAGGT
BsrI 674 ACTGG ACTGG(1/-1)
90 CCAGT
BssKI 54 CCTGG ^CC[ACGT]GG
261 CCTGG
BstNI 54 CCTGG CC^[AT]GG
261 CCTGG
BstXI 392 CCAGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
531 CCAGATCTTTGG
BstYI 253 GGATCC [GA]^GATC[CT]
533 AGATCT
DraII 298 GGGGCCT [GA]G^G[ACGT]CC[CT]
707 GGGGCCC
EarI 502 CTCTTC CTCTTC(1/4)
680 CTCTTC
EcoO109I 298 GGGGCCT [GA]G^G[ACGT]CC[CT]
707 GGGGCCC
EcoRII 54 CCTGG ^CC[AT]GG
261 CCTGG
HinfI 359 GAGTC G^A[ACGT]TC
549 GAATC
Ksp632I 502 CTCTTC CTCTTC(1/4)
680 CTCTTC
MflI 253 GGATCC [GA]^GATC[CT]
533 AGATCT
MvaI 54 CCTGG CC^[AT]GG
261 CCTGG
RsaI 99 GTAC GT^AC
444 GTAC
ScrFI 54 CCTGG CC^[ACGT]GG
261 CCTGG
SfaNI 350 GATGC GCATC(5/9)
518 GCATC
TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^
510 CACTG
XhoII 253 GGATCC [GA]^GATC[CT]
533 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AlwI AvaI BbvI BmyI
BsaJI BsoBI Bsp1286I BstF5I Cac8I
Cfr13I DpnII EcoT14I Fnu4HI FokI
HaeIII HphI ItaI MaeII MboI
MboII MnlI NdeII NlaIII NlaIV
Sau3AI Sau96I StyI TseI Tsp509I