Restriction map for
pFN21AE5293
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeI MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 415 GACGTC GACGT^C
AfaI 135 GTAC GT^AC
ApaI 303 GGGCCC GGGCC^C
BanII 303 GGGCCC G[GA]GC[CT]^C
BbrPI 139 CACGTG CAC^GTG
BbvCI 198 GCTGAGG CCTCAGC(-5/-2)
BsaAI 139 CACGTG [CT]AC^GT[GA]
BsgI 292 GTGCAG GTGCAG(16/14)
BsmAI 418 GTCTC GTCTC(1/5)
BsmBI 417 CGTCTC CGTCTC(1/5)
BsmFI 95 GGGAC GGGAC(10/14)
BsrBI 147 GAGCGG CCGCTC(-3/-3)
BstXI 329 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 199 CTGAG C^T[ACGT]AG
EcoNI 333 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 218 CCAAGG C^C[AT][AT]GG
HaeII 162 GGCGCT [GA]GCGC^[CT]
HphI 370 GGTGA GGTGA(8/7)
KspI 504 CCGCGG CCGC^GG
MaeIII 297 GTCAC ^GT[ACGT]AC
MseI 518 TTAA T^TAA
MspA1I 504 CCGCGG C[AC]G^C[GT]G
PmaCI 139 CACGTG CAC^GTG
PmlI 139 CACGTG CAC^GTG
PpuMI 341 AGGACCT [GA]G^G[AT]CC[CT]
PstI 315 CTGCAG CTGCA^G
RsaI 135 GTAC GT^AC
SacII 504 CCGCGG CCGC^GG
SfcI 315 CTGCAG C^T[GA][CT]AG
SmaI 393 CCCGGG CCC^GGG
StyI 218 CCAAGG C^C[AT][AT]GG
Tru9I 518 TTAA T^TAA
Tsp45I 297 GTCAC ^GT[GC]AC
Tsp509I 520 AATT ^AATT
TspRI 193 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 393 CCCGGG C^CCGGG
XmaI 393 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 35 GACGCC G[GA]^CG[CT]C
415 GACGTC
AvaI 54 CCCGAG C^[CT]CG[GA]G
393 CCCGGG
BmyI 303 GGGCCC G[AGT]GC[ACT]^C
389 GTGCCC
BpmI 232 CTGGAG CTGGAG(16/14)
247 CTGGAG
BsaHI 35 GACGCC G[GA]^CG[CT]C
415 GACGTC
BsoBI 54 CCCGAG C^[CT]CG[GA]G
393 CCCGGG
Bsp1286I 303 GGGCCC G[AGT]GC[ACT]^C
389 GTGCCC
BsrI 132 CCAGT ACTGG(1/-1)
192 CCAGT
BssHII 211 GCGCGC G^CGCGC
263 GCGCGC
DsaI 7 CCACGG C^C[GA][CT]GG
504 CCGCGG
Hin1I 35 GACGCC G[GA]^CG[CT]C
415 GACGTC
MaeII 140 ACGT A^CGT
416 ACGT
MslI 348 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
350 CACAGATGTG
SfaNI 228 GATGC GCATC(5/9)
279 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BbvI BcnI BsaJI BsiYI BslI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI TseI