Restriction map for
pFN21AE5575
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoT22I FbaI FseI HgaI HincII
HindII HindIII HpaI MaeII MamI
MfeI MflI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 788 GGTACC G^GTACC
AcyI 926 GGCGCC G[GA]^CG[CT]C
Alw44I 508 GTGCAC G^TGCAC
AlwI 983 GGATC GGATC(4/5)
ApaLI 508 GTGCAC G^TGCAC
AseI 1299 ATTAAT AT^TAAT
AsnI 1299 ATTAAT AT^TAAT
Asp700I 123 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 788 GGTACC G^GTACC
AviII 548 TGCGCA TGC^GCA
BbeI 926 GGCGCC GGCGC^C
BbsI 378 GAAGAC GAAGAC(2/6)
BfaI 658 CTAG C^TAG
BpuAI 378 GAAGAC GAAGAC(2/6)
BsaHI 926 GGCGCC G[GA]^CG[CT]C
BsaWI 272 TCCGGT [AT]^CCGG[AT]
BsgI 456 CTGCAC GTGCAG(16/14)
BsiEI 648 CGGCCG CG[GA][CT]^CG
BsmBI 309 CGTCTC CGTCTC(1/5)
Bsp1407I 242 TGTACA T^GTACA
BspHI 1265 TCATGA T^CATGA
BsrBI 599 CCGCTC CCGCTC(-3/-3)
BsrDI 1146 CATTGC GCAATG(2/0)
BsrGI 242 TGTACA T^GTACA
BstEII 1086 GGTGACC G^GT[ACGT]ACC
BstPI 1086 GGTGACC G^GT[ACGT]ACC
DpnII 984 GATC ^GATC
DraIII 1243 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 648 CGGCCG C^GGCCG
EclXI 648 CGGCCG C^GGCCG
Eco52I 648 CGGCCG C^GGCCG
EcoNI 135 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1086 GGTGACC G^GT[ACGT]ACC
EcoRV 418 GATATC GAT^ATC
FspI 548 TGCGCA TGC^GCA
Hin1I 926 GGCGCC G[GA]^CG[CT]C
KasI 926 GGCGCC G^GCGCC
KpnI 788 GGTACC GGTAC^C
KspI 796 CCGCGG CCGC^GG
MaeI 658 CTAG C^TAG
MboI 984 GATC ^GATC
MseI 1300 TTAA T^TAA
NaeI 646 GCCGGC GCC^GGC
NarI 926 GGCGCC GG^CGCC
NcoI 197 CCATGG C^CATGG
NdeII 984 GATC ^GATC
NgoMIV 646 GCCGGC G^CCGGC
NspI 1104 GCATGC [GA]CATG^[CT]
PpuMI 814 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1299 ATTAAT AT^TAAT
PvuII 189 CAGCTG CAG^CTG
RcaI 1265 TCATGA T^CATGA
SacI 757 GAGCTC GAGCT^C
SacII 796 CCGCGG CCGC^GG
SapI 126 GCTCTTC GCTCTTC(1/4)
Sau3AI 984 GATC ^GATC
SfoI 926 GGCGCC GGC^GCC
SphI 1104 GCATGC GCATG^C
SspBI 242 TGTACA T^GTACA
Tru9I 1300 TTAA T^TAA
XcmI 1064 CCACGGCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 123 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 797 CGCG CG^CG
1246 CGCG
AocI 32 CCTCAGG CC^T[ACGT]AGG
961 CCTGAGG
ApaI 66 GGGCCC GGGCC^C
498 GGGCCC
AvaI 166 CTCGGG C^[CT]CG[GA]G
751 CCCGAG
AvaII 222 GGTCC G^G[AT]CC
815 GGACC
BcnI 369 CCCGG CC^[GC]GG
884 CCGGG
BsmAI 940 GAGAC GTCTC(1/5)
310 GTCTC
BsoBI 166 CTCGGG C^[CT]CG[GA]G
751 CCCGAG
BspMI 266 ACCTGC ACCTGC(4/8)
1208 ACCTGC
BsrFI 646 GCCGGC [GA]^CCGG[CT]
1090 ACCGGC
BstAPI 1021 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1104 GCATGCACTGC
BstUI 797 CGCG CG^CG
1246 CGCG
BstXI 197 CCATGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCAAAGTGGTGG
Bsu36I 32 CCTCAGG CC^T[ACGT]AGG
961 CCTGAGG
Cfr10I 646 GCCGGC [GA]^CCGG[CT]
1090 ACCGGC
Eco81I 32 CCTCAGG CC^T[ACGT]AGG
961 CCTGAGG
MslI 870 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1296 CACATTAATG
MspA1I 189 CAGCTG C[AC]G^C[GT]G
796 CCGCGG
MvnI 797 CGCG CG^CG
1246 CGCG
NciI 369 CCCGG CC^[GC]GG
884 CCGGG
PstI 52 CTGCAG CTGCA^G
1028 CTGCAG
SfcI 52 CTGCAG C^T[GA][CT]AG
1028 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BalI BanI BanII BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MspI MvaI MwoI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I