Restriction map for
pFN21AE6780
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BbsI
BcgI BciVI BfrI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 400 GTCTAC GT^[AC][GT]AC
AccII 1101 CGCG CG^CG
AcsI 19 GAATTC [GA]^AATT[CT]
AocI 460 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1628 AGCGCT AGC^GCT
ApoI 19 GAATTC [GA]^AATT[CT]
AvaI 345 CCCGGG C^[CT]CG[GA]G
AvrII 1383 CCTAGG C^CTAGG
BanII 132 GAGCCC G[GA]GC[CT]^C
BbeI 442 GGCGCC GGCGC^C
BbrPI 391 CACGTG CAC^GTG
BclI 233 TGATCA T^GATCA
BfaI 1384 CTAG C^TAG
BglII 1275 AGATCT A^GATCT
BlnI 1383 CCTAGG C^CTAGG
BsaAI 391 CACGTG [CT]AC^GT[GA]
BseRI 523 GAGGAG GAGGAG(10/8)
BsoBI 345 CCCGGG C^[CT]CG[GA]G
BspMI 1268 ACCTGC ACCTGC(4/8)
BsrDI 1665 CATTGC GCAATG(2/0)
BsrFI 386 GCCGGC [GA]^CCGG[CT]
BstAPI 802 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1101 CGCG CG^CG
Bsu36I 460 CCTCAGG CC^T[ACGT]AGG
Cfr10I 386 GCCGGC [GA]^CCGG[CT]
Eco47III 1628 AGCGCT AGC^GCT
Eco81I 460 CCTCAGG CC^T[ACGT]AGG
EcoRI 19 GAATTC G^AATTC
FauI 206 CCCGC CCCGC(4/6)
FbaI 233 TGATCA T^GATCA
KasI 442 GGCGCC G^GCGCC
MaeI 1384 CTAG C^TAG
MspA1I 1108 CAGCTG C[AC]G^C[GT]G
MvnI 1101 CGCG CG^CG
NaeI 386 GCCGGC GCC^GGC
NarI 442 GGCGCC GG^CGCC
NgoMIV 386 GCCGGC G^CCGGC
PmaCI 391 CACGTG CAC^GTG
PmlI 391 CACGTG CAC^GTG
PpuMI 1249 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1108 CAGCTG CAG^CTG
SfoI 442 GGCGCC GGC^GCC
SmaI 345 CCCGGG CCC^GGG
SphI 807 GCATGC GCATG^C
TfiI 661 GAATC G^A[AT]TC
Tsp45I 95 GTGAC ^GT[GC]AC
Tsp509I 20 AATT ^AATT
XmaCI 345 CCCGGG C^CCGGG
XmaI 345 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 442 GGCGCC G[GA]^CG[CT]C
1430 GGCGTC
AlwNI 463 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1033 CAGTTCCTG
BamHI 341 GGATCC G^GATCC
488 GGATCC
BglI 613 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
701 GCCTCTATGGC
BsaHI 442 GGCGCC G[GA]^CG[CT]C
1430 GGCGTC
BsmBI 1432 CGTCTC CGTCTC(1/5)
1615 GAGACG
DraII 713 AGGCCCT [GA]G^G[ACGT]CC[CT]
1249 GGGTCCC
EcoNI 651 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1404 CCTTGCTGAGG
EcoO109I 713 AGGCCCT [GA]G^G[ACGT]CC[CT]
1249 GGGTCCC
HgaI 413 GACGC GACGC(5/10)
1431 GCGTC
Hin1I 442 GGCGCC G[GA]^CG[CT]C
1430 GGCGTC
HphI 144 GGTGA GGTGA(8/7)
1497 TCACC
MaeIII 83 GTAAC ^GT[ACGT]AC
95 GTGAC
MslI 231 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
357 CACAGATGTG
NspI 807 GCATGC [GA]CATG^[CT]
1469 GCATGT
PleI 1216 GAGTC GAGTC(4/5)
1388 GAGTC
SexAI 56 ACCTGGT A^CC[AT]GGT
986 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanI BbvCI BbvI
BcnI BmyI BpmI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeII MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI TspRI
TthHB8I XhoII