Restriction map for
pFN21AE5754
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspHI AspI AviII AvrII BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BcnI BglI BlnI BmyI
BpuAI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HapII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MluI MroI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 127 GACGCC G[GA]^CG[CT]C
AflII 432 CTTAAG C^TTAAG
AflIII 363 ACATGT A^C[GA][CT]GT
AhdI 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 120 ATTAAT AT^TAAT
AsnI 120 ATTAAT AT^TAAT
AspEI 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 838 TGGCCA TGG^CCA
BciVI 774 GTATCC GTATCC(6/5)
BclI 99 TGATCA T^GATCA
BfrI 432 CTTAAG C^TTAAG
BlpI 1687 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1687 GCTGAGC GC^T[ACGT]AGC
BsaHI 127 GACGCC G[GA]^CG[CT]C
BseRI 2066 GAGGAG GAGGAG(10/8)
BsmBI 551 GAGACG CGTCTC(1/5)
Bsp1407I 1397 TGTACA T^GTACA
BspLU11I 363 ACATGT A^CATGT
BsrDI 745 GCAATG GCAATG(2/0)
BsrGI 1397 TGTACA T^GTACA
BstAPI 630 GCATTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1687 GCTGAGC GC^T[ACGT]AGC
DraI 1018 TTTAAA TTT^AAA
DraII 2015 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 838 TGGCCA [CT]^GGCC[GA]
Eam1105I 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1657 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2015 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 966 GAATTC G^AATTC
FauI 2021 CCCGC CCCGC(4/6)
FbaI 99 TGATCA T^GATCA
HaeIII 839 GGCC GG^CC
Hin1I 127 GACGCC G[GA]^CG[CT]C
MfeI 769 CAATTG C^AATTG
MluNI 838 TGGCCA TGG^CCA
MscI 838 TGGCCA TGG^CCA
MunI 769 CAATTG C^AATTG
NdeI 1367 CATATG CA^TATG
PpuMI 2015 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 120 ATTAAT AT^TAAT
PstI 1490 CTGCAG CTGCA^G
SspBI 1397 TGTACA T^GTACA
Tsp45I 1036 GTCAC ^GT[GC]AC
XbaI 1158 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 1045 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1495 GAAATTCTTC
AvaI 902 CTCGGG C^[CT]CG[GA]G
1545 CTCGGG
AvaII 1070 GGTCC G^G[AT]CC
2016 GGACC
BamHI 298 GGATCC G^GATCC
897 GGATCC
BglII 373 AGATCT A^GATCT
543 AGATCT
BsaAI 972 TACGTA [CT]AC^GT[GA]
1294 TACGTA
BsaBI 919 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1531 GATGTCAATC
BsmFI 1878 GGGAC GGGAC(10/14)
1957 GGGAC
BsoBI 902 CTCGGG C^[CT]CG[GA]G
1545 CTCGGG
CfoI 1136 GCGC GCG^C
1763 GCGC
Cfr13I 1070 GGTCC G^G[ACGT]CC
2016 GGACC
HhaI 1136 GCGC GCG^C
1763 GCGC
HinP1I 1136 GCGC G^CGC
1763 GCGC
MamI 919 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1531 GATGTCAATC
NspI 363 ACATGT [GA]CATG^[CT]
713 ACATGC
PflMI 174 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1753 CCAGTGATTGG
Sau96I 1070 GGTCC G^G[ACGT]CC
2016 GGACC
SmlI 432 CTTAAG C^T[CT][GA]AG
1081 CTTGAG
SnaBI 972 TACGTA TAC^GTA
1294 TACGTA
Van91I 174 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1753 CCAGTGATTGG
XmnI 1045 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1495 GAAATTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII DraIII EcoRII EcoT14I Fnu4HI
FokI HgaI HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII